Prager M D, Gregg D E, Walter M C
Natl Cancer Inst Monogr. 1978 Dec(49):127-30.
Serum from patients with genitourinary cancer was tested for ability to support transformation of autologous or normal lymphocytes stimulated with ATG. Selected for specificity for T-lymphocytes, ATG at times gave a more sensitive indication of a serum defect than did PHA. Although poor transformability in the presence of patient serum suggests an inhibitor, this test cannot rule out the possibility of a limitation of an essential nutrient usually supplied by serum. Presence of inhibitor(s) was more strongly indicated by contrasting transformation of normal lymphocytes with patient serum against a panel of normal sera. The presence of circulating inhibitor(s) was confirmed by demonstrating depression of transformation of normal lymphocytes in complete medium by chromatographic fractions from patient serum that did not support transformation. In several instances, patient serum was more inhibitory of autologous than normal lymphocytes, which suggested an additional way of examining host immune competence.
对泌尿生殖系统癌症患者的血清进行检测，以评估其支持经抗胸腺细胞球蛋白（ATG）刺激的自体或正常淋巴细胞转化的能力。ATG经筛选具有针对T淋巴细胞的特异性，有时它比植物血凝素（PHA）更能敏感地显示血清缺陷。虽然在患者血清存在的情况下转化能力差提示存在抑制剂，但该试验不能排除血清中通常提供的必需营养素受限的可能性。通过对比正常淋巴细胞与患者血清以及一组正常血清的转化情况，更有力地表明了抑制剂的存在。通过证明来自患者血清的色谱馏分（这些馏分不支持转化）在完全培养基中抑制正常淋巴细胞的转化，证实了循环抑制剂的存在。在一些情况下，患者血清对自体淋巴细胞的抑制作用比对正常淋巴细胞更强，这提示了一种检查宿主免疫能力的额外方法。
