Ge Meng, Li Run-Cheng, Gong Wenjie, Tu Changchun
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130122, China.
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
J Vet Med Sci. 2019 Aug 24;81(8):1173-1176. doi: 10.1292/jvms.18-0482. Epub 2019 Jun 13.
Two commercial porcine reproductive and respiratory syndrome virus (PRRSV) antibody ELISA kits (IDEXX and LSI) are currently in extensive use. To determine which kit is more suitable for the evaluation of HP-PRRSV vaccine efficacy, the two kits were used to test 546 serum samples. The agreement between the results was unsatisfactory, with a kappa statistic of 0.681 and a linear correlation coefficient of 0.665. In tests of samples from experimentally vaccinated and PRRSV-negative herds, IDEXX-ELISA identified antibody-positive conversion earlier and showed a higher specificity compared to LSI-ELISA. The serological profile obtained by neutralization testing was closer to that obtained by IDEXX-ELISA than by LSI-ELISA in the late immunization period. The findings reveal that IDEXX-ELISA is the more suitable for the evaluation of antibody response to HP-PRRSV vaccine and for guiding vaccination strategies.
目前，两种商业化的猪繁殖与呼吸综合征病毒（PRRSV）抗体ELISA试剂盒（IDEXX和LSI）被广泛使用。为了确定哪种试剂盒更适合评估高致病性PRRSV疫苗的效力，使用这两种试剂盒检测了546份血清样本。结果之间的一致性并不理想，kappa统计值为0.681，线性相关系数为0.665。在对来自实验性接种疫苗且PRRSV阴性猪群的样本进行检测时，与LSI-ELISA相比，IDEXX-ELISA更早地识别出抗体阳性转化，并且显示出更高的特异性。在免疫后期，通过中和试验获得的血清学特征与通过IDEXX-ELISA获得的血清学特征比与通过LSI-ELISA获得的血清学特征更接近。研究结果表明，IDEXX-ELISA更适合评估对高致病性PRRSV疫苗的抗体反应并指导疫苗接种策略。
