Sato E, Ishibashi T, Koide S S
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
Biol Reprod. 1987 Sep;37(2):371-6. doi: 10.1095/biolreprod37.2.371.
Mouse follicular oocytes cultured for more than 72 h in modified Krebs-Ringer solution in vitro undergo spontaneous segmentation to form "blastomeres" with or without nuclear fragments or chromatin. Glycosaminoglycans (GAGS) isolated from bovine follicular fluid (bFF) and added to the suspending medium at concentrations of 0.25 mg/ml or greater prevented the occurrence of spontaneous segmentation in isolated cumulus-free mouse oocytes in a dose-dependent manner. The active material was isolated from bFF by chromatography on Dowex 1-X2. The fraction eluted with 0.5 M NaCl possessed the greatest biological activity. Preparations of purified bFF-GAGs and hyaluronic acid and chondroitin sulfate from a commercial source prevented the occurrence of segmentation. Hyaluronic acid was more potent than chondroitin sulfate. The present results show that GAGs prevent segmentation of oocytes in vitro.
在体外改良的krebs - ringer溶液中培养超过72小时的小鼠卵泡卵母细胞会自发分裂，形成带有或不带有核碎片或染色质的“卵裂球”。从牛卵泡液（bFF）中分离出的糖胺聚糖（GAGs），以0.25mg/ml或更高的浓度添加到悬浮培养基中，以剂量依赖的方式阻止了分离的无卵丘小鼠卵母细胞中自发分裂的发生。活性物质通过在Dowex 1 - X2上进行色谱法从bFF中分离出来。用0.5M NaCl洗脱的馏分具有最大的生物活性。从商业来源获得的纯化bFF - GAGs、透明质酸和硫酸软骨素制剂可防止分裂的发生。透明质酸比硫酸软骨素更有效。目前的结果表明，GAGs可防止卵母细胞在体外分裂。
