Department of Biomedical Sciences and Engineering, National Central University, No. 300, Zhongda Rd., Taoyuan City, 32001, Taiwan, ROC.
Department of Chemical and Materials Engineering, National Central University, Taoyuan City, Taiwan, ROC.
Bioprocess Biosyst Eng. 2019 Oct;42(10):1591-1601. doi: 10.1007/s00449-019-02156-z. Epub 2019 Jun 12.
An enhanced greenhouse effect due to high CO emissions has become one of the most concerning issues worldwide. Although plant/algae-mediated approaches have been extensively used for CO segregation in the last decades, these methods are generally aimed at environment protection. In contrast, less attention has been given to CO manipulation that has regrettably caused a decrease in the commercial availability of the associated technologies. To generate a system for practical use, a synthetic fluorocarbon photobioreactor system (FCPBRS) consisting of a CO isolation unit, a gas modulation unit, an O collection unit, and a microalgal culture chamber was developed in this study. After injecting a 60%-N/40%-CO gas mixture into the CO isolation unit for 10 days, the results showed that the FCPBRS enabled a > 93% CO separation efficiency using a fluorocarbon liquid FC-40 as the CO adsorbent. In addition, the growth rate of Nannochloropsis oculata was significantly enhanced when cultured with 20 mL min of the FC-40 flow containing 2% CO throughout the time course, resulting in 4.7-, 4.6-, and 4.5-fold (P < 0.05 for each) increases in biomass, total lipid, and eicosapentaenoic acid yields, respectively, compared to the aerated group without FC-40. Moreover, approximately 1600 mL of photosynthetic O with a ~ 80% collection efficiency was obtained in the O collection unit within 10 days of FCPBRS operation. These outcomes indicate that the FCPBRS may provide a feasible means to simultaneously achieve CO isolation, O collection, and enhanced microalgae bioproductions.
由于高浓度 CO 排放导致的温室效应增强已成为全球最关注的问题之一。尽管在过去几十年中，植物/藻类介导的方法已被广泛用于 CO 分离，但这些方法通常旨在保护环境。相比之下，对于 CO 的操纵关注较少，这导致与 CO 相关的技术的商业可用性降低。为了生成实用系统，本研究开发了一种由 CO 隔离单元、气体调制单元、O 收集单元和微藻培养室组成的合成氟碳光生物反应器系统（FCPBRS）。将 60%-N/40%-CO 气体混合物注入 CO 隔离单元 10 天后，结果表明，FCPBRS 使用氟碳液体 FC-40 作为 CO 吸附剂实现了 >93%的 CO 分离效率。此外，当用含有 2%CO 的 20 mL min 的 FC-40 流量培养 Nannochloropsis oculata 时，其生长速度明显提高，与不含 FC-40 的通气组相比，生物量、总脂质和二十碳五烯酸产量分别增加了 4.7、4.6 和 4.5 倍（P<0.05）。此外，在 FCPBRS 运行 10 天内，O 收集单元中获得了约 1600 mL 具有 ~80%收集效率的光合 O。这些结果表明，FCPBRS 可能为同时实现 CO 分离、O 收集和增强微藻生物生产提供一种可行的方法。
