赖氨酸特异性去甲基化酶1（LSD1）的上调通过促进上皮-间质转化（EMT）来促进胃癌的迁移和侵袭。
Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT.
作者信息
Zhang Jin, Zhao Donghui, Li Qingjun, Du Xiuluan, Liu Yanxiang, Dai Xin, Hong Lianqing
机构信息
Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China,
Department of Neurology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China.
出版信息
Cancer Manag Res. 2019 May 15;11:4481-4491. doi: 10.2147/CMAR.S186649. eCollection 2019.
BACKGROUND
Gastric cancer (GC) is a common malignant tumor of the digestive system. In addition, GC metastasis is an extremely complicated process. A previous study has found that lysine-specific demethylase 1 (LSD1) is abnormal expression in a variety of cancers and its overexpression correlates with aggressive disease and poor outcome.
METHODS
qRT-PCR and Western blot assays were used to assess the expression of LSD1 in GC tissue samples and cell lines. Colony formation assay, CCK-8 assay, scratch-wound assay and transwell invasion, were performed to determine the effect of LSD1 on cell proliferation and migration as well as invasion in GC.
RESULTS
Our results show that LSD1 was up-regulated in GC tumor tissues and cell lines, and high expression level of LSD1 was found to be positively correlated with tumor size, lymph node metastasis and pathological grade. Moreover, LSD1 promoted cell proliferation, migration and invasion of GC. In addition, LSD1 regulated E-cadherin expression through demethylating H3K4me2, thereby promoting EMT in GC.
CONCLUSION
Our work indicated that LSD1 may be used as a potential target of gastric cancer.
背景
胃癌（GC）是消化系统常见的恶性肿瘤。此外，胃癌转移是一个极其复杂的过程。先前的一项研究发现，赖氨酸特异性去甲基化酶1（LSD1）在多种癌症中表达异常，其过表达与疾病侵袭性和不良预后相关。
方法
采用qRT-PCR和蛋白质免疫印迹法检测LSD1在胃癌组织样本和细胞系中的表达。进行集落形成试验、CCK-8试验、划痕试验和Transwell侵袭试验，以确定LSD1对胃癌细胞增殖、迁移和侵袭的影响。
结果
我们的结果表明，LSD1在胃癌肿瘤组织和细胞系中上调，并且发现LSD1的高表达水平与肿瘤大小、淋巴结转移和病理分级呈正相关。此外，LSD1促进胃癌细胞的增殖、迁移和侵袭。此外，LSD1通过去甲基化H3K4me2调节E-钙黏蛋白的表达，从而促进胃癌中的上皮-间质转化（EMT）。
结论
我们的研究表明，LSD1可能作为胃癌的潜在靶点。
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