Pang Hai-Lin, Zhao Qian-Qian, Ma Yu, Song Yong-Li, Min Jie, Lu Jian-Rong, Li Hang, Zhao Da-Qing
Department of Otolaryngology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi'an 710038, China.
Department of Oncology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi'an 710038, China.
Stem Cells Int. 2019 May 5;2019:2139814. doi: 10.1155/2019/2139814. eCollection 2019.
Adipose-derived stem cells (ADSCs) are multipotent and have received increasing attention for their applications in medicine. Cell-based therapies are optimal for diseases with loss or damage to tissues or organs. ADSCs and bone marrow mesenchymal stem cells (BMSCs) can differentiate into many cell lineages. Because of their advantages in accessibility and volume, ADSCs are regarded as a desirable alternative to BMSCs. In this study, we focused on the chondrocytic differentiation potential of ADSCs and the underlying mechanism. We found that the long noncoding RNA H19 plays an important role in this process. Overexpression of H19 in ADSCs induced differentiation towards chondrocytes. H19 is abundantly expressed during embryonic development and downregulated after birth, implying its regulatory role in determining cell fate. However, in our experiments, H19 exerted its regulatory function during cartilage differentiation of ADSCs through competing miRNA regulation of STAT2.
脂肪来源干细胞（ADSCs）具有多能性，并且因其在医学中的应用而受到越来越多的关注。基于细胞的疗法对于组织或器官丧失或受损的疾病是最佳的。ADSCs和骨髓间充质干细胞（BMSCs）可以分化为许多细胞谱系。由于ADSCs在获取和数量方面的优势，它们被视为BMSCs的理想替代物。在本研究中，我们聚焦于ADSCs的软骨细胞分化潜能及其潜在机制。我们发现长链非编码RNA H19在此过程中发挥重要作用。ADSCs中H19的过表达诱导其向软骨细胞分化。H19在胚胎发育期间大量表达，出生后下调，这暗示了其在决定细胞命运中的调节作用。然而，在我们的实验中，H19通过竞争性miRNA调节STAT2在ADSCs的软骨分化过程中发挥其调节功能。
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