State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, P. R. China.
Small. 2019 Aug;15(31):e1901907. doi: 10.1002/smll.201901907. Epub 2019 Jun 13.
Poor post-traumatic wound healing can affect the normal function of damaged tissues and organs. For example, poor healing of corneal epithelial injuries may lead to permanent visual impairment. It is of great importance to find a therapeutic way to promote wound closure. Tetrahedral framework nucleic acids (tFNAs) are new promising nanomaterials, which can affect the biological behavior of cells. In the experiment, corneal wound healing is used as an example to explore the effect of tFNAs on wound healing. Results show that the proliferation and migration of human corneal epithelial cells are enhanced by exposure to tFNAs in vitro, possibly relevant to the activation of P38 and ERK1/2 signaling pathway. An animal model of corneal alkali burn is established to further identify the facilitation effect of tFNAs on corneal wound healing in vivo. Clinical evaluations and histological analyses show that tFNAs can improve the corneal transparency and accelerate the re-epithelialization of wounds. Both in vitro and in vivo experiments show that tFNAs can play a positive role in corneal epithelial wound healing.
创伤后愈合不良会影响受损组织和器官的正常功能。例如，角膜上皮损伤愈合不良可能导致永久性视力损害。因此，寻找一种促进伤口闭合的治疗方法非常重要。四面体框架核酸（tFNAs）是一种新型的有前途的纳米材料，可影响细胞的生物学行为。在实验中，以角膜伤口愈合为例，探讨 tFNAs 对伤口愈合的影响。结果表明，tFNAs 体外可促进人角膜上皮细胞的增殖和迁移，这可能与 P38 和 ERK1/2 信号通路的激活有关。建立角膜碱烧伤动物模型，进一步鉴定 tFNAs 对体内角膜伤口愈合的促进作用。临床评估和组织学分析表明，tFNAs 可以提高角膜透明度并加速伤口的再上皮化。体外和体内实验均表明，tFNAs 可在角膜上皮伤口愈合中发挥积极作用。
