Furukawa J, Shimano T, Inaji H, Saito M, Matsuura N, Monden T, Mori T
Second Department of Surgery, Osaka University Medical School, Japan.
Clin Chim Acta. 1987 Sep 30;168(2):219-30. doi: 10.1016/0009-8981(87)90291-9.
A pancreatic cancer-related antigen (PCRA) has been identified, isolated and characterized from liver metastases of pancreatic carcinoma. The purified PCRA was homogeneous by polyacrylamide disc gel electrophoresis, had an Mr of about 500,000, consisted of 30% carbohydrate and 70% protein, and migrated in the alpha 2-beta region in electrophoresis. In gel immunoprecipitation the antiserum against PCRA reacted with extracts of pancreatic cancer and spleen, but neither with normal human serum nor with normal pancreas, liver, lung, large and small intestine extracts. It clearly differed from other known pancreatic tumor-associated antigens.
一种与胰腺癌相关的抗原（PCRA）已从胰腺癌肝转移灶中被鉴定、分离和表征。纯化后的PCRA经聚丙烯酰胺圆盘凝胶电泳显示为均一性，分子量约为500,000，由30%的碳水化合物和70%的蛋白质组成，在电泳中迁移至α2-β区域。在凝胶免疫沉淀中，抗PCRA抗血清与胰腺癌和脾脏提取物发生反应，但与正常人血清以及正常胰腺、肝脏、肺、大肠和小肠提取物均无反应。它明显不同于其他已知的胰腺肿瘤相关抗原。
