Shimano T, Mori T, Kitada M, Maruyama H, Kosaki G
Ann N Y Acad Sci. 1983;417:97-104. doi: 10.1111/j.1749-6632.1983.tb32853.x.
The same antigenic substance as pancreatic cancer-associated antigen (PCAA), sharing an identical immunogenicity with Gelder's POA, was isolated and purified from normal colonic mucosa. Purified colonic PCAA (PCAAc) is a glycoprotein with a molecular weight of approximately 600,000; it consists of 30% carbohydrates and 70% peptides, and appears to be an N-glycosidic glycoprotein. Antiserum raised against purified PCAAc showed one fused line with our original anti-PCAA antiserum to ascites fluid from patients with pancreatic cancer and extract from normal colonic mucosa. Circulating PCAAc levels assayed by rocket immunoelectrophoresis showed significant elevation in sera of patients with carcinoma of the pancreas. It is concluded that PCAA might be a new tumor-associated antigen which is produced ectopically during oncogenesis.
从正常结肠黏膜中分离并纯化出了与胰腺癌相关抗原（PCAA）相同的抗原物质，它与盖尔德氏胰腺癌相关抗原（POA）具有相同的免疫原性。纯化后的结肠PCAA（PCAAc）是一种糖蛋白，分子量约为600,000；它由30%的碳水化合物和70%的肽组成，似乎是一种N-糖苷糖蛋白。用纯化的PCAAc制备的抗血清与我们原来针对胰腺癌患者腹水和正常结肠黏膜提取物的抗PCAA抗血清显示出一条融合线。通过火箭免疫电泳检测的循环PCAAc水平在胰腺癌患者血清中显著升高。结论是，PCAA可能是一种新的肿瘤相关抗原，在肿瘤发生过程中异位产生。
