Guin Sailen, Saravanan Murugan, Chowdhury Goutam, Pazhani Gururaja Perumal, Ramamurthy Thandavarayan, Chandra Das Suresh
Joyrampur Bidyabhavan, Hogalberia, 741122, Nadia, West Bengal, India.
Veterinary Dispensary, Arunthavapuram, Thanjore, Tamil Nadu, 614401, India.
Heliyon. 2019 May 17;5(5):e01743. doi: 10.1016/j.heliyon.2019.e01743. eCollection 2019 May.
The role of in causing diarrhoeal disease is well known. However, phenotypic and genetic traits of this pathogen isolated from diverse sources have not been investigated in detail. In this study, we have screened samples from diarrhoeal cases (2603), brackish water fish (301) and aquatic environments (115) and identified in 29 (1.1%), 171 (56.8%) and 43 (37.4%) samples, respectively. Incidence of pathogenic with virulence encoding thermostable-direct haemolysin gene () was detected mostly in fishes (19.3%) and waters (15.6%) than clinical samples (1.04%). The pandemic strain marker genes ( and PGS-sequences) have been detected relatively more in water (6%) and fish (5%) samples than in clinical samples (0.7%). Majority of the isolates from clinical cases and fish samples (26.3%) belonged to classical pandemic serovars (O3:K6). In addition, several newly recognised pandemic serovars have also been identified. Pulsed field-gel electrophoresis (PFGE) analysis showed clonal relatedness (60-85%) of from different sources. The study observation revealed that the brackish water fishes and water bodies may act as a reservoir of pathogenic . Emergence of several new serovars of pandemic signifies the changing phenotypic characteristics of the pathogen.
[病原体名称]在引起腹泻疾病中的作用是众所周知的。然而，从不同来源分离出的这种病原体的表型和遗传特征尚未得到详细研究。在本研究中，我们对腹泻病例（2603份）、咸水鱼（301份）和水生环境（115份）的样本进行了筛查，分别在29份（1.1%）、171份（56.8%）和43份（37.4%）样本中鉴定出了[病原体名称]。携带编码耐热直接溶血素基因（[基因名称]）的致病[病原体名称]的发生率在鱼类（19.3%）和水体（15.6%）中检测到的大多比临床样本（1.04%）中更高。大流行菌株标记基因（[基因名称]和PGS序列）在水样（6%）和鱼样（5%）中检测到的相对比临床样本（0.7%）中更多。从临床病例和鱼样本中分离出的大多数[病原体名称]菌株（26.3%）属于经典大流行血清型（O3:K6）。此外，还鉴定出了几种新确认的大流行血清型。脉冲场凝胶电泳（PFGE）分析显示来自不同来源的[病原体名称]具有克隆相关性（60 - 85%）。该研究观察结果表明，咸水鱼和水体可能作为致病[病原体名称]的储存库。几种大流行新血清型的出现表明该病原体的表型特征正在发生变化。
