Sun Lin, Guo Yujie, He Peng, Xu Xiaoyan, Zhang Xiong, Wang Haiyang, Tang Tian, Zhou Wei, Xu Ping, Xie Peng
Neuroscience Center, Key Laboratory of Neurobiology of Chongqing, Chongqing, China.
Department of Pain, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Genes Dis. 2019 Apr 17;6(2):147-158. doi: 10.1016/j.gendis.2019.04.002. eCollection 2019 Jun.
Borna disease virus 1 (BoDV-1) is neurotropic prototype of Bornaviruses causing neurological diseases and maintaining persistent infection in brain cells of mammalian species. Long non-coding RNA (lncRNA) is transcript of more than 200 nucleotides without protein-coding function regulating various biological processes as proliferation, apoptosis, cell migration and viral infection. However, regulatory of lncRNAs in BoDV-1 infection remains unknown. To identify differential expression profiles and predict functions of lncRNA in BoDV-1 infection, microarray data showed that 3528 lncRNAs and 2661 lncRNAs were differentially expressed in Strain V and Hu-H1 BoDV-infected groups compared with control groups, respectively. Gene Ontology (GO) and pathway analysis suggested that differential lncRNAs may be involved in regulation of metabolic, biological regulation, cellular process, endocytosis, viral infections and cell adhesion processes, cancer in both BoDV-infected strains. ENSMUST00000128469 was found down-regulated in both BoDV-infected groups compared with control groups consistent with microarray (p < 0.05). ceRNA analysis indicated possible interaction networks as ENSMUST00000128469/miR-22-5p, miR-206-3p, miR-302b-5p, miR-302c-3p, miR-1a-3p/Igf1. Igf1 was found up-regulated in both BoDV-infected groups compared with control groups (p < 0.05). Possible functions of predicted target mRNAs and miRNAs of ENSMUST00000128469 were involved in cell proliferation, transcriptional misregulation and proteoglycan pathways enriched in cancer. lncRNA may be involved in regulation of Hu-H1 inhibited cell proliferation and promoted apoptosis through NF-kB, JNK/MAPK signaling, BCL2 and CDK6/E2F1 pathways different from Strain V. Possible interaction networks as ENSMUST00000128469/miR-22-5p, miR-206-3p, miR-302b-5p, miR-302c-3p, miR-1a-3p/Igf1 may involve in regulation of cell proliferation, apoptosis, and cancer.
博尔纳病病毒1（BoDV-1）是博尔纳病毒的嗜神经原型，可引发神经疾病并在哺乳动物的脑细胞中维持持续性感染。长链非编码RNA（lncRNA）是一种转录本，其长度超过200个核苷酸，没有蛋白质编码功能，但可调节多种生物学过程，如增殖、凋亡、细胞迁移和病毒感染。然而，lncRNAs在BoDV-1感染中的调控作用仍不清楚。为了鉴定lncRNA在BoDV-1感染中的差异表达谱并预测其功能，微阵列数据显示，与对照组相比，在V株和Hu-H1株BoDV感染组中分别有3528个和2661个lncRNAs差异表达。基因本体论（GO）和通路分析表明，差异lncRNAs可能参与了两种BoDV感染株的代谢、生物调节、细胞过程、内吞作用、病毒感染和细胞黏附过程以及癌症的调控。与对照组相比，在两个BoDV感染组中均发现ENSMUST00000128469表达下调，这与微阵列结果一致（p<0.05）。ceRNA分析表明存在可能的相互作用网络，如ENSMUST00000128469/miR-22-5p、miR-206-3p、miR-302b-5p、miR-302c-3p、miR-1a-3p/Igf1。与对照组相比，在两个BoDV感染组中均发现Igf1表达上调（p<0.05）。ENSMUST00000128469预测的靶mRNA和miRNA的可能功能涉及细胞增殖、转录失调和癌症中富集的蛋白聚糖途径。lncRNA可能通过与V株不同的NF-kB、JNK/MAPK信号通路、BCL2和CDK6/E2F1途径参与Hu-H1抑制细胞增殖和促进凋亡的调控。可能的相互作用网络，如ENSMUST00000128469/miR-22-5p、miR-206-3p、miR-302b-5p、miR-302c-3p、miR-1a-3p/Igf1可能参与细胞增殖、凋亡和癌症的调控。
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