斑马鱼中同源转录因子编码基因的特征分析

Characterization of paralogous transcription factor encoding genes in zebrafish.

作者信息

Nittoli Valeria, Fortunato Antonio Emidio, Fasano Giulia, Coppola Ugo, Gentile Alessandra, Maiella Sylvie, Langellotto Fernanda, Porreca Immacolata, De Paolo Raffaella, Marino Rita, Fiengo Marcella, Donizetti Aldo, Aniello Francesco, Kondo Takashi, Ristoratore Filomena, Canzoniero Lorella M T, Duboule Denis, Wilson Stephen W, Sordino Paolo

机构信息

Biology and Evolution of Marine Organisms, Zoological Station Anton Dohrn, 80121 Naples, Italy.

Immunrise Technologies, 75005 Paris, France.

出版信息

Gene X. 2019 Jun;2:100011. doi: 10.1016/j.gene.2019.100011.

DOI:10.1016/j.gene.2019.100011

PMID:31193955

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6543554/

Abstract

The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis in vertebrates. Reasoning that zebrafish genes and are orthologs of mouse , we studied their genomic environment and developmental expression. Evolutionary analyses indicate the zebrafish genes as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount mRNA hybridization of transcripts in zebrafish embryos reveals novel expression domains, confirms those previously known, and suggests sub-functionalization of paralogs. Using genetic mutants and pharmacological inhibitors, we investigate the role of signaling pathways on the expression of zebrafish genes in developing somites. In identifying putative functional role(s) of zebrafish genes, we hypothesized that they encode transcription factors that coordinate growth and innervation of somitic muscles.

摘要

配对型同源结构域转录因子Uncx参与脊椎动物胚胎发育的多个过程。基于斑马鱼基因和是小鼠直系同源基因的推断，我们研究了它们的基因组环境和发育表达。进化分析表明，斑马鱼基因是硬骨鱼特异性全基因组复制产生的旁系同源基因。对斑马鱼胚胎中转录本进行的全胚胎mRNA杂交揭示了新的表达结构域，证实了先前已知的结构域，并表明旁系同源基因发生了亚功能化。我们使用基因突变体和药物抑制剂，研究了信号通路对斑马鱼基因在发育中的体节表达的作用。在确定斑马鱼基因的假定功能作用时，我们假设它们编码协调体节肌肉生长和神经支配的转录因子。