Schauerte H E, van Eeden F J, Fricke C, Odenthal J, Strähle U, Haffter P
Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/III, Germany.
Development. 1998 Aug;125(15):2983-93. doi: 10.1242/dev.125.15.2983.
Sonic hedgehog (Shh) is a secreted protein that is involved in the organization and patterning of several tissues in vertebrates. We show that the zebrafish sonic-you (syu) gene, a member of a group of five genes required for somite patterning, is encoding Shh. Embryos mutant for a deletion of syu display defects in patterning of the somites, the lateral floor plate cells, the pectoral fins, the axons of motorneurons and the retinal ganglion cells. In contrast to mouse embryos lacking Shh activity, syu mutant embryos do form medial floor plate cells and motorneurons. Since ectopic overexpression of shh in zebrafish embryos does not induce ectopic medial floor plate cells, we conclude that shh is neither required nor sufficient to induce this cell type in the zebrafish.
音猬因子（Shh）是一种分泌蛋白，参与脊椎动物多种组织的组织形成和模式化过程。我们发现，斑马鱼的音猬因子类似基因（syu）是体节模式化所需的一组五个基因中的一员，它编码Shh。syu基因缺失的突变胚胎在体节、外侧底板细胞、胸鳍、运动神经元轴突和视网膜神经节细胞的模式化方面表现出缺陷。与缺乏Shh活性的小鼠胚胎不同，syu突变胚胎确实形成了内侧底板细胞和运动神经元。由于在斑马鱼胚胎中异位过表达shh不会诱导异位内侧底板细胞的形成，我们得出结论，shh在斑马鱼中既不是诱导这种细胞类型所必需的，也不是充分的。
