Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, 71-210 Szczecin, Poland.
VitroLive Fertility Clinic in Szczecin, 70-483 Szczecin, Poland.
Int J Environ Res Public Health. 2019 Jun 5;16(11):2004. doi: 10.3390/ijerph16112004.
Because the assessment of sperm DNA fragmentation (SDF) plays a key role in male fertility, our study was designed to find the relationships between SDF and standard semen parameters. The receiver operating characteristic (ROC) curve showed that 18% SDF is a prognostic parameter for discriminating between men with normal and abnormal standard semen parameters (n = 667). Men with > 18% SDF had significantly lower quality semen, a higher prevalence of abnormal semen characteristics, and a higher odds ratio for abnormal semen parameters compared to men with ≤ 18% SDF. An ROC analysis provided predictive values for age and semen parameters to distinguish between men with SDF > 18% and men with ≤ 18% SDF. SDF was positively correlated with male age and teratozoospermia index but negatively with sperm concentration, total number of spermatozoa, sperm morphology, progressive motility, and vitality. Our study shows that 18% SDF has a predictive value for distinguishing between men with normal and abnormal semen characteristics. Men with >18% SDF have a higher risk for abnormal semen parameters, while age and obtained semen parameters have a predictive value for SDF. There is a relationship between SDF and conventional sperm characteristics, and thus, SDF can be incorporated into male fertility assessment.
由于精子 DNA 碎片化（SDF）的评估在男性生育力中起着关键作用，因此我们的研究旨在寻找 SDF 与标准精液参数之间的关系。受试者工作特征（ROC）曲线显示，18%的 SDF 是区分正常和异常标准精液参数男性的预后参数（n=667）。与 SDF≤18%的男性相比，SDF＞18%的男性的精液质量明显更低，异常精液特征的患病率更高，异常精液参数的优势比更高。ROC 分析提供了年龄和精液参数的预测值，以区分 SDF＞18%的男性和 SDF≤18%的男性。SDF 与男性年龄和畸形精子指数呈正相关，但与精子浓度、总精子数、精子形态、前向运动和活力呈负相关。我们的研究表明，18%的 SDF 对区分正常和异常精液特征具有预测价值。SDF＞18%的男性异常精液参数的风险更高，而年龄和获得的精液参数对 SDF 具有预测价值。SDF 与常规精子特征之间存在关系，因此 SDF 可纳入男性生育力评估。
