沙特男性精子 DNA 碎片化与生活方式因素和精液参数的关系及其对 ICSI 结局的影响。
Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome.
机构信息
Zoology Department, King Saud University, Riyadh, Saudi Arabia.
Assisted Reproductive Technology Laboratories, Thuriah Medical Center, Riyadh, Saudi Arabia.
出版信息
Reprod Biol Endocrinol. 2018 May 19;16(1):49. doi: 10.1186/s12958-018-0369-3.
BACKGROUND
Male factor infertility is quite common as 30-50% of infertility cases are due to sperm defects. The high sperm DNA fragmentation is one of the causes of male factor infertility. Many factors cause sperm DNA fragmentation and could be testicular or post-testicular. The purpose of this study was to assess relationships among sperm DNA fragmentation, lifestyle factors and semen values of Saudi men and to determine impact of sperm DNA fragmentation on ICSI cycle outcome.
METHODS
The duration of this study was from January 2015 to June 2016. The cases with female factor infertility were excluded. In total 94 couples were selected for investigation. The study parameters were male age, body mass index, smoking, semen values, % sperm DNA fragmentation, fertilization rate and pregnancy outcome. The ICSI procedure was performed in all patients per standard protocol. The semen samples were grouped based on % sperm DNA fragmentation into < 15%, 15-30 and > 30% which corresponded to low, moderate and high sperm DNA fragmentation, respectively.
RESULTS
There was no difference in ICSI outcome in low and moderate sperm DNA fragmentation, however, in high sperm DNA fragmentation no patient achieved pregnancy. In this study, 53.19% Saudi men had low, 32.98% moderate and 13.83% high DFI. Semen volume, sperm morphology and fertilization rate did not show any correlation trend with DNA fragmentation, however, sperm concentration and motility were negatively correlated in all DFI categories. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.
CONCLUSIONS
The results of this study indicated that 14% Saudi men had high DNA fragmentation. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.
背景
男性因素不孕相当常见，因为 30-50%的不孕病例是由于精子缺陷引起的。精子 DNA 碎片化是男性因素不孕的原因之一。许多因素会导致精子 DNA 碎片化，这些因素可能是睾丸内的或睾丸后的。本研究的目的是评估沙特男性的精子 DNA 碎片化与生活方式因素和精液值之间的关系，并确定精子 DNA 碎片化对 ICSI 周期结局的影响。
方法
本研究的持续时间为 2015 年 1 月至 2016 年 6 月。排除了女性因素不孕的病例。共有 94 对夫妇被选入研究。研究参数包括男性年龄、体重指数、吸烟、精液值、精子 DNA 碎片化百分比、受精率和妊娠结局。所有患者均按标准方案行 ICSI 程序。精液样本根据精子 DNA 碎片化百分比分为<15%、15-30%和>30%，分别对应低、中和高精子 DNA 碎片化。
结果
低和中度精子 DNA 碎片化的 ICSI 结局无差异，但高精子 DNA 碎片化的患者无一例妊娠。在本研究中，53.19%的沙特男性精子 DNA 碎片化程度低，32.98%的男性精子 DNA 碎片化程度中等，13.83%的男性精子 DNA 碎片化程度高。精液量、精子形态和受精率与 DNA 碎片化均无相关趋势，但在所有 DNA 碎片化类别中，精子浓度和活力呈负相关。体重指数与中度 DNA 碎片化类别呈正相关，吸烟与低度 DNA 碎片化类别呈正相关。年龄与中度和高度 DNA 碎片化类别呈正相关。
结论
本研究结果表明，14%的沙特男性精子 DNA 碎片化程度较高。体重指数与中度 DNA 碎片化类别呈正相关，吸烟与低度 DNA 碎片化类别呈正相关。年龄与中度和高度 DNA 碎片化类别呈正相关。
Zotero 插件