RASSF1A 甲基化诊断乳腺癌的价值：一项荟萃分析。
Diagnostic value of RASSF1A methylation for breast cancer: a meta-analysis.
机构信息
Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun 130021, Jilin, China.
School of Mathematics and Statistics, Northeast Normal University, Changchun 130000, Jilin, China.
出版信息
Biosci Rep. 2019 Jun 28;39(6). doi: 10.1042/BSR20190923.
BACKGROUND
Numerous studies reported that RAS-association domain family 1 isoform A () methylation might act as diagnostic biomarker for breast cancer (BC), this meta-analysis aimed to evaluate the value of methylation for diagnosing BC.
METHODS
Such databases as PubMed, Cochrane Library and Web of Science databases were searched for literatures until May 2019. A meta-analysis was performed utilizing STATA and Revman softwares. Furthermore, subgroup analysis was adopted to determine likely sources of heterogeneity.
RESULTS
Totally 19 literatures with 1849 patients and 1542 controls were included in the present study. Sensitivity, specificity, diagnostic odds ratio (DOR) and the area under the summary receiver operating characteristic curve (AUC) of methylation for diagnosing BC were 0.49, 0.95, 19.0 and 0.83, respectively. The sensitivity (0.54 vs 0.43), DOR (30.0 vs 10.0) and AUC (0.84 vs 0.81) of methylation in Caucasian were higher than other ethnicities. The sensitivity (0.64 vs 0.57), DOR (21.0 vs 14.0) and AUC (0.89 vs 0.86) of methylation-specific PCR (MSP) were superior to other methods (q-MSP, OS-MSP and MethyLight). The sensitivity, DOR and AUC of serum methylation vs methylation in other samples (tissue or plasma) were 0.55 vs 0.40, 22.0 vs 14.0 and 0.86 vs 0.74, respectively.
CONCLUSIONS
methylation might be a potential diagnostic biomarker for BC. Considering its low sensitivity and high specificity, it should combine with others to upgrade the sensitivity. Besides, under such conditions, MSP detection, serum methylation and Caucasian are shown to be more effective and suitable for diagnosing BC.
背景
大量研究表明 RAS 关联结构域家族 1 同种型 A（）甲基化可能作为乳腺癌（BC）的诊断生物标志物，本荟萃分析旨在评估 甲基化用于诊断 BC 的价值。
方法
检索 PubMed、Cochrane 图书馆和 Web of Science 数据库等数据库，检索截至 2019 年 5 月的文献。使用 STATA 和 Revman 软件进行荟萃分析。此外，还采用亚组分析来确定异质性的可能来源。
结果
共纳入 19 项研究，共纳入 1849 例患者和 1542 例对照。用于诊断 BC 的 甲基化的灵敏度、特异度、诊断优势比（DOR）和汇总受试者工作特征曲线下面积（AUC）分别为 0.49、0.95、19.0 和 0.83。在白人中，甲基化的灵敏度（0.54 对 0.43）、DOR（30.0 对 10.0）和 AUC（0.84 对 0.81）均高于其他种族。甲基化特异性 PCR（MSP）的灵敏度（0.64 对 0.57）、DOR（21.0 对 14.0）和 AUC（0.89 对 0.86）优于其他方法（q-MSP、OS-MSP 和 MethyLight）。血清 甲基化与其他样本（组织或血浆）中 甲基化的灵敏度、DOR 和 AUC 分别为 0.55 对 0.40、22.0 对 14.0 和 0.86 对 0.74。
结论
甲基化可能是 BC 的潜在诊断生物标志物。鉴于其灵敏度低、特异性高，应与其他方法联合提高灵敏度。此外，在这种情况下，MSP 检测、血清 甲基化和白种人显示出更有效和更适合诊断 BC。
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