利用快速魔角旋转 NMR 技术对固态蛋白质的结构和动力学进行精确的距离测量。
Exact distance measurements for structure and dynamics in solid proteins by fast-magic-angle-spinning NMR.
机构信息
Faculty for Chemistry and Pharmacy, Ludwig-Maximilians-University Munich, Butenandtstr. 5-13, 81377 Munich, Germany.
Department of Chemistry, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA.
出版信息
Chem Commun (Camb). 2019 Jul 14;55(55):7899-7902. doi: 10.1039/c9cc02317h. Epub 2019 Jun 14.
Abstract
Fast-magic-angle-spinning solid-state NMR is a developing technique for determination of protein structure and dynamics. Proton-proton correlations usually lead to rough distance restraints, a serious hurdle towards high-resolution structures. Analogous to the "eNOE" concept in solution, an integrative approach for more accurate restraints enables improved structural accuracy with minimal analytical effort.
摘要
快速魔角旋转固态 NMR 是一种用于测定蛋白质结构和动态的发展中的技术。质子-质子相关通常会导致粗略的距离约束，这是朝向高分辨率结构的一个严重障碍。类似于溶液中的“eNOE”概念，一种综合的方法可以获得更准确的约束，从而在最小的分析工作量下提高结构准确性。
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