Du Cailian, Yang Bo, Wu Jianhui, Ali Shaukat
Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, South China Agricultural University, Guangzhou 510642, China.
Insects. 2019 Jun 13;10(6):168. doi: 10.3390/insects10060168.
(Bagnall) is one of the most harmful pests of leguminous plants. In order to expand our knowledge on the infection of by entomopathogenic fungi, two newly identified isolates of (Zare & Gams) were tested for their pathogenicity against . Both isolates of (SCAUDCL-38 and SCAUDCL-56) were isolated from soil and were identified by morphological and molecular analyses. The adult females of were treated with five different concentrations (1 × 10, 1 × 10, 1 × 10, 1 × 10, and 1 × 10 conidia/mL) of the isolates. Our results revealed 76.25% and 57.5% mortality of after five days of treatment with 1 × 10 conidia/mL of SCAUDCL-38 and SCAUDCL-56, respectively. The median lethal concentrations (LC) of SCAUDCL-38 and SCAUDCL-56 calculated through linear regression analysis after five days of fungal treatment of were 1.9 × 10 and 1.5 × 10 conidia/mL, respectively, whereas the median lethal time (LT) observed for 1 × 10 conidia/mL of SCAUDCL-38 and SCAUDCL-56 were 3.52 days and 4.9 days, respectively. isolates SCAUDCL-38 and SCAUDCL-56 are highly pathogenic strains of . These findings offer valuable information on the development and commercialization of alternative control measures against .
（巴格纳尔）是豆科植物最具危害性的害虫之一。为了拓展我们对昆虫病原真菌感染（该害虫）的认识，对两种新鉴定的（某种真菌，此处原文未明确写出具体名称，仅用括号表示）分离株（扎雷和甘姆斯）针对（该害虫）的致病性进行了测试。两种（该真菌）分离株（SCAUDCL - 38和SCAUDCL - 56）均从土壤中分离得到，并通过形态学和分子分析进行了鉴定。用该分离株的五种不同浓度（1×10、1×10、1×10、1×10和1×10分生孢子/毫升）处理（该害虫）的成年雌性个体。我们的结果显示，用1×10分生孢子/毫升的SCAUDCL - 38和SCAUDCL - 56处理五天后，（该害虫）的死亡率分别为76.25%和57.5%。在对（该害虫）进行真菌处理五天后，通过线性回归分析计算得出的SCAUDCL - 38和SCAUDCL - 56的半数致死浓度（LC）分别为1.9×10和1.5×10分生孢子/毫升，而对于1×10分生孢子/毫升的SCAUDCL - 38和SCAUDCL - 56观察到的半数致死时间（LT）分别为3.52天和4.9天。（该真菌）分离株SCAUDCL - 38和SCAUDCL - 56是（该真菌）的高致病性菌株。这些发现为针对（该害虫）的替代控制措施开发和商业化提供了有价值的信息。
