Tripathi B J, Geanon J D, Tripathi R C
Department of Ophthalmology and Visual Science, University of Chicago, IL.
Ophthalmology. 1987 Nov;94(11):1434-8. doi: 10.1016/s0161-6420(87)33278-6.
The authors examined various structures of human and rhesus monkey eyes for the presence of tissue plasminogen activator (t-PA) by using the peroxidase-antiperoxidase immunohistochemical technique with a monoclonal antibody specific for human t-PA. Positive staining for t-PA was observed both intracellularly and in the extracellular matrix of many tissues in both species. The tissues which stained intensely for t-PA included the corneal endothelium, corneal epithelium, trabecular meshwork, lens epithelium, peripheral vitreous, uveal tract, inner retina, and all vascular endothelia. The apparent minor difference in staining intensity between human and monkey eyes may be related to the time-dependent degradation of t-PA, to variations in the tissue content of t-PA, or to the difference in animal species. The discussion includes a consideration of the fibrinolytic activity of t-PA and of its emerging role in the destructive remodeling of the extracellular matrix in various ocular structures.
作者使用针对人组织型纤溶酶原激活剂（t-PA）的单克隆抗体，采用过氧化物酶-抗过氧化物酶免疫组织化学技术，检测了人眼和恒河猴眼的各种结构中是否存在t-PA。在这两个物种的许多组织的细胞内和细胞外基质中均观察到t-PA的阳性染色。t-PA染色强烈的组织包括角膜内皮、角膜上皮、小梁网、晶状体上皮、周边玻璃体、葡萄膜、视网膜内层以及所有血管内皮。人眼和猴眼在染色强度上的明显细微差异可能与t-PA的时间依赖性降解、t-PA的组织含量变化或动物物种差异有关。讨论内容包括对t-PA的纤溶活性及其在各种眼部结构的细胞外基质破坏性重塑中新兴作用的思考。
