Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
Biol Reprod. 2019 Aug 1;101(2):416-425. doi: 10.1093/biolre/ioz100.
Long noncoding RNAs (lncRNAs) are important regulators that have multiple functions in a variety of biological processes. However, the contributions of lncRNAs to follicle-stimulating hormone (FSH) secretion remain largely unknown. In this study, we first identified a novel lncRNA, lncRNA-m433s1, as an intergenic lncRNA located in the cytoplasm. We next used MS2-RIP assays to demonstrate that lncRNA-m433s1 interacted with miR-433. Furthermore, we detected the levels of lncRNA-m433s1, miR-433, and Fshβ expression, FSH concentrations, and apoptosis upon overexpression and knockdown of lncRNA-m433s1, revealing that lncRNA-m433s1 upregulated Fshβ expression. Globally, lncRNA-m433s1 reduced the inhibitory effect of miR-433 on Fshβ and further regulated FSH secretion as a competing endogenous RNA (ceRNA) by sponging miR-433. This ceRNA model will provide novel insight into the regulatory mechanisms of lncRNAs associated with rat reproduction.
长链非编码 RNA（lncRNAs）是重要的调控因子，在多种生物学过程中具有多种功能。然而，lncRNAs 对卵泡刺激素（FSH）分泌的贡献在很大程度上仍然未知。在这项研究中，我们首先鉴定了一种新型 lncRNA，lncRNA-m433s1，作为位于细胞质中的基因间 lncRNA。接下来，我们使用 MS2-RIP 测定来证明 lncRNA-m433s1 与 miR-433 相互作用。此外，我们检测了过表达和敲低 lncRNA-m433s1 后 lncRNA-m433s1、miR-433 和 Fshβ 表达、FSH 浓度和细胞凋亡的水平，发现 lncRNA-m433s1 上调了 Fshβ 的表达。总的来说，lncRNA-m433s1 降低了 miR-433 对 Fshβ 的抑制作用，并进一步作为竞争性内源性 RNA（ceRNA）通过海绵 miR-433 调节 FSH 分泌。该 ceRNA 模型将为与大鼠生殖相关的 lncRNA 调节机制提供新的见解。
