Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science, Wuhan, China.
Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Hubei Academy of Agricultural Science, Wuhan, China.
Cell Cycle. 2021 May;20(9):927-942. doi: 10.1080/15384101.2021.1911102. Epub 2021 Apr 12.
Follicle development is a vital factor which determines the reproductive performance of poultry. Long noncoding RNAs (lncRNAs) have been reported to maintain animal reproductive function and play key roles in ovarian development and hormone secretion. But the regulatory mechanism of lncRNAs in duck follicle development has seldom been reported. In this study, to better explore the molecular mechanism of follicle development in ducks, the follicular lncRNA was sequenced and analyzed. A total of 9,551 lncRNAs were predicted in the duck follicles. Four hundred and forty-five lncRNAs were differentially expressed between the white follicles and yellow follicles. The results of our studies showed that lnc_13814 promoted cell apoptosis in duck GCs. Furthermore, the bioinformatics analysis results demonstrated that lnc_13814 was involved in a lncRNA-miRNA-mRNA coexpression network and it was observed to sponge two follicle-related miRNAs by a luciferase activity assay. Moreover, we found that overexpression of lnc_13814 significantly increased DNA damage inducible transcript 3 (DDIT3) expression and downregulated GCs apoptosis. Finally, we found that lnc_13814 directly binds to and inhibits apla-mir-145-4; then, lnc_13814 increases the expression of DDIT3 and up-regulates GCs apoptosis. Taken together, our findings demonstrate that lncRNAs have potential effects on duck ovarian follicles and lncRNAs may represent a new approach to understand follicular development.
卵泡发育是决定家禽繁殖性能的重要因素。长链非编码 RNA（lncRNA）已被报道维持动物生殖功能，并在卵巢发育和激素分泌中发挥关键作用。但 lncRNA 在鸭卵泡发育中的调控机制很少有报道。在这项研究中，为了更好地探索鸭卵泡发育的分子机制，对卵泡中的 lncRNA 进行了测序和分析。在鸭卵泡中预测到了 9551 个 lncRNA。在白卵泡和黄卵泡之间有 445 个 lncRNA 表达差异。我们的研究结果表明，lnc_13814 促进了鸭 GC 的细胞凋亡。此外，生物信息学分析结果表明，lnc_13814 参与了 lncRNA-miRNA-mRNA 的共表达网络，并通过荧光素酶活性测定观察到它能海绵两个与卵泡相关的 miRNA。此外，我们发现 lnc_13814 的过表达显著增加了 DNA 损伤诱导转录物 3（DDIT3）的表达，并下调了 GC 的凋亡。最后，我们发现 lnc_13814 直接结合并抑制 apla-mir-145-4；然后，lnc_13814 增加了 DDIT3 的表达并上调了 GC 的凋亡。总之，我们的研究结果表明，lncRNA 对鸭卵巢卵泡具有潜在的影响，lncRNA 可能代表一种理解卵泡发育的新方法。
