Aung Shuh-Wen, Abu Kasim Noor Hayaty, Ramasamy Thamil Selvee
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Regenerative Dentistry Research Group, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Methods Mol Biol. 2019;2045:323-335. doi: 10.1007/7651_2019_242.
The therapeutic potential of human mesenchymal stromal stem cells (hMSCs) for cell-based therapeutic is greatly influenced by the in vitro culture condition including the culture conditions. Nevertheless, there are many technical challenges needed to be overcome prior to the clinical use including the quantity, quality, and heterogeneity of the cells. Therefore, it is necessary to develop a stem cell culture procedure or protocol for cell expansion in order to generate reproducible and high-quality cells in accordance with good manufacturing practice for clinical and therapeutic purposes. Here we assessed the MSCs characteristic of human Wharton's jelly mesenchymal stromal cells in in vitro culture according to the criteria established by the International Society for Cellular Therapy. Besides, the viability of the WJMSCs was determined in order to increase the confidence that the cells are employed to meet the therapeutic efficacy.
人间充质基质干细胞（hMSCs）用于基于细胞的治疗的治疗潜力受到包括培养条件在内的体外培养条件的极大影响。然而，在临床应用之前，仍有许多技术挑战需要克服，包括细胞的数量、质量和异质性。因此，有必要开发一种用于细胞扩增的干细胞培养程序或方案，以便根据临床和治疗目的的良好生产规范生成可重复且高质量的细胞。在此，我们根据国际细胞治疗协会制定的标准，评估了体外培养的人脐带华通氏胶间充质基质细胞的间充质干细胞特性。此外，还测定了WJMSCs的活力，以增强对所使用细胞达到治疗效果的信心。
