Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, Xi'an, 710055, Shaanxi, P. R. China.
Agency for Science, Technology, and Research (A*STAR), Singapore, 138673, Singapore.
J Microbiol. 2019 Sep;57(9):812-820. doi: 10.1007/s12275-019-9036-4. Epub 2019 Jun 14.
Sanghuangporus sanghuang is a well-known pharmacodynamic and economically important edible fungus associated with mulberry (Morus spp.). A distinctly new exopolysaccharide (EPS), designated SHP-2 was obtained from S. sanghuang P0988 broth, and its structure and anti-aging prosperity were characterized. SHP-2 was found to be composed of a back-bone of →4)-β-Manp-(1→4)-α-Araf-(1→3,4)-α-Glcp(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→6)-α-Galp-(1→4)-β-Manp-(1→ and five branches, including four α-D-Glcp-(1→ and one α-D-Manp-(1→SHP-2 was shown to increase antioxidant enzyme activities including catalase (CAT) and superoxide dismutase (SOD) activities, as well as trolox equivalent antioxidant (TEAC) capacity in serum of mice pre-treated with D-Gal, while reducing lipofuscin levels. SHP-2 exerted a favorable influence on immune organ coefficients and ameliorated the histopathological hepatic lesions and apoptosis in hepatocytes of D-galactose-aged mice almost in a dose-dependent manner. Using the same analytical methods, on comparison with previously studied EPS compounds (i.e. SHP-1), SHP-2 was found to have more complex structure, larger molecule weight, and different anti-aging properties. The results presented here suggest that not only does EPS bioactivity vary with respect to molecular structures and molecule weight, but that multiple structures with different activity can be expressed by a single fungal strain. These results may help understanding the anti-aging prosperity of these polysaccharides for use in health foods or dietary supplements.
裂褶菌是一种与桑树（桑属）相关的具有明显药效和经济重要性的食用真菌。从裂褶菌 P0988 发酵液中获得了一种新型胞外多糖（EPS），命名为 SHP-2，并对其结构和抗衰老活性进行了表征。SHP-2 由→4)-β-Manp-(1→4)-α-Araf-(1→3,4)-α-Glcp(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→6)-α-Galp-(1→4)-β-Manp-(1→和五个支链组成，其中包括四个α-D-Glcp-(1→和一个α-D-Manp-(1→。SHP-2 能够提高抗氧化酶（CAT 和 SOD）的活性，增加血清中 trolox 等效抗氧化能力（TEAC），改善 D-半乳糖预处理小鼠的脂褐素水平。SHP-2 对免疫器官系数也有有利影响，几乎呈剂量依赖性地改善了衰老小鼠肝组织的病理损伤和肝细胞凋亡。采用相同的分析方法，与先前研究的 EPS 化合物（即 SHP-1）相比，SHP-2 具有更复杂的结构、更大的分子量和不同的抗衰老特性。这些结果表明，EPS 的生物活性不仅与分子结构和分子量有关，而且单个真菌菌株可以表达具有不同活性的多种结构。这些结果可能有助于理解这些多糖的抗衰老活性，用于保健食品或膳食补充剂。
