School of Life Sciences, Anhui University, Hefei, China.
Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, China.
J Sci Food Agric. 2021 Feb;101(3):1009-1020. doi: 10.1002/jsfa.10709. Epub 2020 Aug 26.
Sanghuang mushrooms are medicinal fungi widely used in eastern Asia. In this study, the antioxidant and anti-inflammatory activity of a novel extracellular polysaccharopeptide, sanghuang extracellular polysaccharopeptide (SePSP) was investigated. The SePSP was purified from the submerged fermentation broth of a sanghuang mycelium, Sanghuangporus lonicericola strain CBS17, which was isolated from a wild sanghuang fruiting body.
The SePSP was extracted using an ethanol precipitation procedure, followed by diethylaminoethanol (DEAE) anion-exchange and size-exclusion chromatography. The mass ratio of the polysaccharide and peptide components in the purified SePSP was approximately 4.87:1. By determining its free radical scavenging abilities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), the hydroxyl free radical, and the superoxide anion free radical, as well as its total reducing power, SePSP was shown to have strong concentration-dependent antioxidant activity in vitro. Further, SePSP effectively alleviated dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice. Administration of 200 mg kg SePSP by gavage for 7 days prevented body weight loss; significantly reduced the mRNA levels of proinflammatory cytokines, including TNF-α and IL-1β; increased mRNA level of the anti-inflammatory cytokine IL-10 in the colon, and decreased the malondialdehyde concentration from 6.42 to 4.82 μmol L in the blood in UC mice.
The SePSP had strong concentration-dependent antioxidant activity in vitro and effectively alleviated DSS-induced UC in mice. The in vivo therapeutic efficacy in DSS-induced UC may be mediated by modulating the expression of inflammatory cytokines and inhibiting oxidative stress. The findings provide a scientific rationale for the use of bioactive nutraceuticals from sanghuang mushrooms to develop functional foods for the prevention and treatment of UC. © 2020 Society of Chemical Industry.
桑黄蘑菇是一种在东亚广泛使用的药用真菌。在这项研究中，研究了一种新型胞外多糖肽，即桑黄胞外多糖肽（SePSP）的抗氧化和抗炎活性。SePSP 是从桑黄菌丝体（分离自野生桑黄子实体的 Sanghuangporus lonicericola 菌株 CBS17）的液体发酵液中提取的。
SePSP 是通过乙醇沉淀法提取的，然后通过二乙氨基乙基（DEAE）阴离子交换和分子筛层析进行纯化。纯化的 SePSP 中多糖和肽成分的质量比约为 4.87:1。通过使用 2,2-二苯基-1-苦基肼（DPPH）、羟基自由基和超氧阴离子自由基测定其自由基清除能力，以及总还原能力，结果表明 SePSP 在体外具有很强的浓度依赖性抗氧化活性。此外，SePSP 可有效缓解葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）。通过灌胃给予 200mg/kg SePSP 7 天可防止体重减轻；显著降低 UC 小鼠促炎细胞因子 TNF-α 和 IL-1β 的 mRNA 水平；增加结肠中抗炎细胞因子 IL-10 的 mRNA 水平，并使 UC 小鼠血液中的丙二醛浓度从 6.42μmol/L 降低至 4.82μmol/L。
SePSP 在体外具有很强的浓度依赖性抗氧化活性，并能有效缓解 DSS 诱导的 UC 小鼠。DSS 诱导的 UC 中的体内治疗效果可能是通过调节炎症细胞因子的表达和抑制氧化应激来介导的。这些发现为利用桑黄蘑菇中的生物活性营养保健品开发用于预防和治疗 UC 的功能性食品提供了科学依据。 © 2020 英国化学学会。
