Department of Civil Engineering, Anna University Regional Campus, Tirunelveli, Tamil Nadu, India.
Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.
Bioresour Technol. 2019 Oct;289:121624. doi: 10.1016/j.biortech.2019.121624. Epub 2019 Jun 8.
This study involves the application of new phase separated biological pretreatment (PSBP) strategy on microalgal biomass using the nickel nanoparticle induced cellulase secreting bacterial disintegration. Particularly, interest was focussed on cell wall weakening (CWW) of microalgae biomass besides the cell disintegration (CD) and release of organics. During CWW, protein, carbohydrate, cellulose, hemicellulose and DNA were used as evaluation indexes. Similarly, during CD, soluble chemical oxygen demand was used as evaluation index to assess the disintegration effect. A higher CWW was achieved at nickel nanoparticle (Np) dosage of 0.004 g/g SS. During CD, a clear demarcation in biomass solubilisation was achieved by PSBP (36%) than the sole biological pretreatment -BP (24%). The biomethanogenesis test results showed that enhanced methane production of 411 mL/g COD was achieved by PSBP than BP. Energy analysis showed that a higher net energy production of 6.467 GJ/d was achieved by PSBP.
本研究采用镍纳米粒子诱导产纤维素酶细菌解体的新相分离生物预处理（PSBP）策略，将其应用于微藻生物质。特别关注微藻生物质的细胞壁弱化（CWW），除了细胞解体（CD）和有机物的释放。在 CWW 过程中，使用蛋白质、碳水化合物、纤维素、半纤维素和 DNA 作为评估指标。同样，在 CD 过程中，使用可溶化学需氧量作为评估指标来评估解体效果。在镍纳米粒子（Np）剂量为 0.004 g/g SS 时，实现了更高的 CWW。在 CD 过程中，PSBP（36%）比单一生物预处理 -BP（24%）更明显地实现了生物质的溶解。生物甲烷生成测试结果表明，PSBP 比 BP 可实现 411 mL/g COD 的增强甲烷生成。能量分析表明，PSBP 可实现更高的净能量产生，为 6.467 GJ/d。
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