Konja Daniels, Luo Cuiting, Sun Wai Yan, Yang Kangmin, Man Andy W C, Xu Aimin, Vanhoutte Paul M, Wang Yu
The State Key Laboratory of Pharmaceutical Biotechnology and the Department of Pharmacology and Pharmacy, University of Hong Kong.
The State Key Laboratory of Pharmaceutical Biotechnology and the Department of Pharmacology and Pharmacy, University of Hong Kong;
J Vis Exp. 2019 Jun 3(148). doi: 10.3791/59688.
Altered vascular tone responsiveness to pathophysiological stimuli contributes to the development of a wide range of cardiovascular and metabolic diseases. Endothelial dysfunction represents a major culprit for the reduced vasodilatation and enhanced vasoconstriction of arteries. Adipose (fat) tissues surrounding the arteries play important roles in the regulation of endothelium-dependent relaxation and/or contraction of the vascular smooth muscle cells. The cross-talks between the endothelium and perivascular adipose tissues can be assessed ex vivo using mounted blood vessels by a wire myography system. However, optimal settings should be established for arteries derived from animals of different species, ages, genetic backgrounds and/or pathophysiological conditions.
血管对病理生理刺激的反应性改变会导致多种心血管和代谢疾病的发生。内皮功能障碍是动脉血管舒张功能降低和血管收缩增强的主要原因。动脉周围的脂肪组织在调节血管平滑肌细胞的内皮依赖性舒张和/或收缩中起重要作用。内皮与血管周围脂肪组织之间的相互作用可以通过线肌电图系统使用安装好的血管在体外进行评估。然而，对于来自不同物种、年龄、遗传背景和/或病理生理状况的动物的动脉，应建立最佳设置。
