Department of Pathology, Tianjin First Center Hospital, Tianjin 300192, China.
Biomed Res Int. 2019 May 8;2019:9085435. doi: 10.1155/2019/9085435. eCollection 2019.
Interferon-induced transmembrane protein 1 (IFITM1), a 17-kDa membrane protein, is generally known as a modulator in many cellular functions. Recent studies showed overexpression of IFITM1 in cancers and relationship between IFITM1 overexpression and tumor progression. However, the role of IFITM1 in lung cancer remains unclear. Here, we presented the overexpression of IFITM1 in lung cancer tissues and cell lines A549 and H460 using quantitative Real-Time RT-PCR. assay indicated IFITM1 silencing inhibited lung cancer cell proliferation, migration, and invasion. Further, assay showed that IFITM1 silencing markedly suppressed cell growth and metastasis of lung cancer in tumor-bearing BALB/c nude mice. Mechanistically, we found that IFITM1 silencing significantly alleviated the protein levels of -catenin, cyclin D1, and c-Mycin lung cancer cells and tumor samples. Taken together, our study revealed the role of IFITM1 as a tumor promoter during lung cancer development and the possible molecular mechanism.
干扰素诱导跨膜蛋白 1（IFITM1）是一种 17kDa 的膜蛋白，通常被认为是许多细胞功能的调节剂。最近的研究表明，IFITM1 在癌症中的过表达与肿瘤进展之间存在关系。然而，IFITM1 在肺癌中的作用尚不清楚。在这里，我们使用定量实时 RT-PCR 显示 IFITM1 在肺癌组织和细胞系 A549 和 H460 中的过表达。实验表明，IFITM1 沉默抑制肺癌细胞的增殖、迁移和侵袭。进一步的实验表明，IFITM1 沉默显著抑制荷瘤 BALB/c 裸鼠肺癌细胞的生长和转移。机制上，我们发现 IFITM1 沉默显著减轻了肺癌细胞和肿瘤样本中 -catenin、cyclin D1 和 c-Myc 的蛋白水平。综上所述，我们的研究揭示了 IFITM1 在肺癌发展过程中作为肿瘤促进因子的作用及其可能的分子机制。
