Department of Biology, School of Basic Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.
Burn Research Center, Iran University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2019 Sep 1;136:616-624. doi: 10.1016/j.ijbiomac.2019.06.061. Epub 2019 Jun 15.
Cold Atmospheric Plasma (CAP) is used as a promising method in surface modification for immobilization of chondroitin sulfate functional biomacromolecules on PCL nanofibrous substrates for cartilage tissue engineering. The GAG-grafted scaffolds are able to successfully support the attachment and proliferation of mesenchymal stem cells (MSCs). The seeded scaffolds show the chondro-differentiation of MSCs during a 21-days cell culture in a non-differential medium. Expression of SOX9, Collagen10 and Collagen2 proved the chondro-inductive effect of GAG-grafted scaffolds. Besides, no external chondro-genic differential agent was used in the differentiation of MSCs to chondrocyte. The cells passed the last phase of chondrogenesis after 14 days of incubation. Thus, the GAG-fabricated fibrous scaffolds using CAP are potential candidates for cartilage tissue engineering.
冷等离体等离子体 (CAP) 被用作表面改性的一种有前途的方法，用于将硫酸软骨素功能生物大分子固定在 PCL 纳米纤维基质上，用于软骨组织工程。接枝 GAG 的支架能够成功地支持间充质干细胞 (MSCs) 的附着和增殖。在非分化培养基中进行 21 天细胞培养期间，接种的支架显示 MSC 的软骨分化。SOX9、Collagen10 和 Collagen2 的表达证明了接枝 GAG 的支架的软骨诱导作用。此外，在 MSC 向软骨细胞的分化过程中没有使用外部的软骨诱导分化剂。细胞经过 14 天的孵育后通过了软骨形成的最后阶段。因此，使用 CAP 制造的 GAG 纤维支架是软骨组织工程的潜在候选材料。
