硫酸软骨素固定化的聚己内酯纳米纤维增强间充质干细胞的软骨分化。
Chondroitin sulfate immobilized PCL nanofibers enhance chondrogenic differentiation of mesenchymal stem cells.
机构信息
Department of Biology, School of Basic Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.
Burn Research Center, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Int J Biol Macromol. 2019 Sep 1;136:616-624. doi: 10.1016/j.ijbiomac.2019.06.061. Epub 2019 Jun 15.
Cold Atmospheric Plasma (CAP) is used as a promising method in surface modification for immobilization of chondroitin sulfate functional biomacromolecules on PCL nanofibrous substrates for cartilage tissue engineering. The GAG-grafted scaffolds are able to successfully support the attachment and proliferation of mesenchymal stem cells (MSCs). The seeded scaffolds show the chondro-differentiation of MSCs during a 21-days cell culture in a non-differential medium. Expression of SOX9, Collagen10 and Collagen2 proved the chondro-inductive effect of GAG-grafted scaffolds. Besides, no external chondro-genic differential agent was used in the differentiation of MSCs to chondrocyte. The cells passed the last phase of chondrogenesis after 14 days of incubation. Thus, the GAG-fabricated fibrous scaffolds using CAP are potential candidates for cartilage tissue engineering.
冷等离体等离子体 (CAP) 被用作表面改性的一种有前途的方法，用于将硫酸软骨素功能生物大分子固定在 PCL 纳米纤维基质上，用于软骨组织工程。接枝 GAG 的支架能够成功地支持间充质干细胞 (MSCs) 的附着和增殖。在非分化培养基中进行 21 天细胞培养期间，接种的支架显示 MSC 的软骨分化。SOX9、Collagen10 和 Collagen2 的表达证明了接枝 GAG 的支架的软骨诱导作用。此外，在 MSC 向软骨细胞的分化过程中没有使用外部的软骨诱导分化剂。细胞经过 14 天的孵育后通过了软骨形成的最后阶段。因此，使用 CAP 制造的 GAG 纤维支架是软骨组织工程的潜在候选材料。
Zotero 插件