UNIROUEN, INSERM UMR 1073 "Nutrition, Inflammation and Gut-Brain Axis", Normandie University, 76183 Rouen, France.
Institute of Research and Innovation in Biomedicine (IRIB), UNIROUEN, Normandie University, 76183 Rouen, France.
Nutrients. 2019 Jun 15;11(6):1348. doi: 10.3390/nu11061348.
During activity-based anorexia (ABA) in mice, enhanced paracellular permeability and reduced protein synthesis have been shown in the colon while the gut-brain axis has received increasing attention in the regulation of intestinal and mood disorders that frequently occur during anorexia nervosa, a severe eating disorder for which there is no specific treatment. In the present study, we assessed the effects of oral glutamine (Gln) or branched-chain amino acids (BCAA) supplementation during ABA to target intestinal functions, body composition and feeding behavior.
C57BL/6 male mice were randomized in Control (CTRL) and ABA groups. After ABA induction, mice received, or not, either 1% Gln or 2.5% BCAA (Leu, Ile, Val) for one week in drinking water.
Neither Gln nor BCAA supplementation affected body weight and body composition, while only Gln supplementation slightly increased food intake. ABA mice exhibited increased paracellular permeability and reduced protein synthesis in the colonic mucosa. Oral Gln restored colonic paracellular permeability and protein synthesis and increased the mucin-2 mRNA level, whereas BCAA did not affect colonic parameters.
In conclusion, oral Gln specifically improves colonic response during ABA. These data should be further confirmed in AN patients.
在活动相关厌食症（ABA）的小鼠模型中，结肠的细胞旁通透性增加和蛋白质合成减少已被证实，而肠道-大脑轴在调节经常发生于神经性厌食症（一种严重的进食障碍，目前尚无特异性治疗方法）的肠道和情绪障碍方面受到了越来越多的关注。在本研究中，我们评估了 ABA 期间口服补充谷氨酰胺（Gln）或支链氨基酸（BCAA）对肠道功能、身体成分和摄食行为的影响。
将 C57BL/6 雄性小鼠随机分为对照组（CTRL）和 ABA 组。ABA 诱导后，小鼠在饮用水中接受或不接受 1% Gln 或 2.5% BCAA（亮氨酸、异亮氨酸、缬氨酸）补充 1 周。
Gln 或 BCAA 补充均不影响体重和身体成分，而 Gln 补充仅略微增加了食物摄入量。ABA 小鼠的结肠黏膜细胞旁通透性增加和蛋白质合成减少。口服 Gln 可恢复结肠细胞旁通透性和蛋白质合成，并增加粘蛋白-2 mRNA 水平，而 BCAA 则不影响结肠参数。
总之，口服 Gln 可特异性改善 ABA 期间的结肠反应。这些数据应在 AN 患者中进一步确认。
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