AMPK对肠上皮顶端连接和屏障功能的调节作用
AMPK in regulation of apical junctions and barrier function of intestinal epithelium.
作者信息
Zhu Mei-Jun, Sun Xiaofei, Du Min
机构信息
a School of Food Science , Washington State University , Pullman , WA, USA.
b Department of Animal Sciences , Washington State University , Pullman , WA, USA.
出版信息
Tissue Barriers. 2018;6(2):1-13. doi: 10.1080/21688370.2018.1487249. Epub 2018 Aug 21.
Abstract
Gut epithelium covers the inner layer of the gastrointestinal tract and provides a physical barrier to separate the host from its external environment, and its barrier function is critical for maintaining host health. AMP-activated protein kinase (AMPK) as a master regulator of energy metabolism plays a critical role in epithelial barrier function. AMPK activation promotes epithelial differentiation and facilitates cell polarity establishment, both of which strengthen epithelial barrier. In addition, AMPK promotes the assembly of tight junctions and adherens junctions by direct phosphorylation of proteins composing apical junctions, junctional anchors, and cytoskeletons. Pharmacological and nutraceutical compounds, as well as physiological states triggering AMPK activation strengthen epithelial barrier function. This review summarized recent progress in delineating the regulatory roles of AMPK in apical junction formation and barrier function of intestinal epithelium.
摘要
肠道上皮覆盖胃肠道内层，提供物理屏障以将宿主与其外部环境分隔开，其屏障功能对于维持宿主健康至关重要。AMP激活的蛋白激酶（AMPK）作为能量代谢的主要调节因子，在上皮屏障功能中起关键作用。AMPK激活促进上皮分化并有助于细胞极性建立，这两者都能加强上皮屏障。此外，AMPK通过直接磷酸化构成顶端连接、连接锚定和细胞骨架的蛋白质来促进紧密连接和黏附连接的组装。药理和营养化合物以及触发AMPK激活的生理状态可增强上皮屏障功能。本综述总结了在阐明AMPK在肠道上皮顶端连接形成和屏障功能中的调节作用方面的最新进展。
相似文献
1
AMPK in regulation of apical junctions and barrier function of intestinal epithelium.AMPK对肠上皮顶端连接和屏障功能的调节作用
Tissue Barriers. 2018;6(2):1-13. doi: 10.1080/21688370.2018.1487249. Epub 2018 Aug 21.
2
Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers.丁酸盐通过激活Caco-2细胞单层中的AMP活化蛋白激酶促进紧密连接组装，从而增强肠道屏障。
J Nutr. 2009 Sep;139(9):1619-25. doi: 10.3945/jn.109.104638. Epub 2009 Jul 22.
3
Reciprocal Association between the Apical Junctional Complex and AMPK: A Promising Therapeutic Target for Epithelial/Endothelial Barrier Function?顶端连接复合体与 AMPK 的相互关系：上皮/内皮屏障功能的有前途的治疗靶点？
Int J Mol Sci. 2019 Nov 29;20(23):6012. doi: 10.3390/ijms20236012.
4
A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway.一种益生元果寡糖通过 AMPK 依赖途径促进肠道上皮细胞的紧密连接组装。
Biomed Pharmacother. 2020 Sep;129:110415. doi: 10.1016/j.biopha.2020.110415. Epub 2020 Jun 27.
5
AMPK Activation Promotes Tight Junction Assembly in Intestinal Epithelial Caco-2 Cells.AMPK 激活促进肠道上皮细胞 Caco-2 中的紧密连接组装。
Int J Mol Sci. 2019 Oct 18;20(20):5171. doi: 10.3390/ijms20205171.
6
An agonist of a zinc-sensing receptor GPR39 enhances tight junction assembly in intestinal epithelial cells via an AMPK-dependent mechanism.锌敏感受体 GPR39 的激动剂通过 AMPK 依赖的机制增强肠道上皮细胞的紧密连接组装。
Eur J Pharmacol. 2019 Jan 5;842:306-313. doi: 10.1016/j.ejphar.2018.10.038. Epub 2018 Oct 26.
7
Implications of AMPK in the Formation of Epithelial Tight Junctions.AMPK 在上皮紧密连接形成中的作用。
Int J Mol Sci. 2018 Jul 13;19(7):2040. doi: 10.3390/ijms19072040.
8
Rho kinase regulates tight junction function and is necessary for tight junction assembly in polarized intestinal epithelia.Rho激酶调节紧密连接功能，并且对于极化肠道上皮细胞中紧密连接的组装是必需的。
Gastroenterology. 2001 Sep;121(3):566-79. doi: 10.1053/gast.2001.27060.
9
GPR40 receptor activation promotes tight junction assembly in airway epithelial cells via AMPK-dependent mechanisms.GPR40受体激活通过AMPK依赖机制促进气道上皮细胞紧密连接组装。
Tissue Barriers. 2018;6(2):1-12. doi: 10.1080/21688370.2018.1480741. Epub 2018 Aug 29.
10
Regulation of epithelial tight junction assembly and disassembly by AMP-activated protein kinase.AMP 激活的蛋白激酶对上皮紧密连接组装和解聚的调节
Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):819-22. doi: 10.1073/pnas.0610157104. Epub 2007 Jan 4.
引用本文的文献
1
MG4244 Protects Against Metabolic and Inflammatory Stress in .MG4244可抵御……中的代谢和炎症应激。 （原文不完整，翻译至此）
Foods. 2025 Jun 5;14(11):1995. doi: 10.3390/foods14111995.
2
Nature's Remedies: Exploring the Potential of Propolis to Alleviate Non-Motor Manifestations of Parkinson's Disease.大自然的疗法：探索蜂胶缓解帕金森病非运动症状的潜力。
Molecules. 2025 Apr 8;30(8):1672. doi: 10.3390/molecules30081672.
3
In Vitro Protective Effects of a Standardized Extract of (L.) Mill. Cladodes and L. Leaves Against Indomethacin-Induced Intestinal Epithelial Cell Injury.（L.）Mill. 叶状枝和L. 叶的标准化提取物对吲哚美辛诱导的肠上皮细胞损伤的体外保护作用。
Antioxidants (Basel). 2024 Dec 10;13(12):1507. doi: 10.3390/antiox13121507.
4
Integrated multi-omics analyses revealed the association between rheumatoid arthritis and colorectal cancer: MYO9A as a shared gene signature and an immune-related therapeutic target.整合多组学分析揭示类风湿关节炎与结直肠癌的关联：肌球蛋白IXA 作为共同的基因特征和免疫相关的治疗靶点。
BMC Cancer. 2024 Jun 10;24(1):714. doi: 10.1186/s12885-024-12466-5.
5
Arabinogalactan Alleviates Lipopolysaccharide-Induced Intestinal Epithelial Barrier Damage through Adenosine Monophosphate-Activated Protein Kinase/Silent Information Regulator 1/Nuclear Factor Kappa-B Signaling Pathways in Caco-2 Cells.阿拉伯半乳糖聚糖通过腺苷酸活化蛋白激酶/沉默信息调节因子 1/核因子 κB 信号通路减轻脂多糖诱导的 Caco-2 细胞肠上皮屏障损伤。
Int J Mol Sci. 2023 Oct 19;24(20):15337. doi: 10.3390/ijms242015337.
6
Dietary yeast glycoprotein supplementation improves the growth performance, intestinal health and disease resistance of largemouth bass () fed low-fishmeal diets.日粮酵母糖蛋白补充料改善了摄食低鱼粉饲料的大口黑鲈的生长性能、肠道健康和抗病力。
Front Immunol. 2023 May 15;14:1164087. doi: 10.3389/fimmu.2023.1164087. eCollection 2023.
7
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.奶源性细胞外囊泡保护肠道-肝脏轴中肠道屏障的完整性。
Sci Adv. 2023 Apr 14;9(15):eade5041. doi: 10.1126/sciadv.ade5041. Epub 2023 Apr 12.
8
Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells.多物种益生菌菌株混合物通过调节脂多糖刺激的Caco-2细胞中的炎症和紧密连接来增强肠道屏障功能。
Microorganisms. 2023 Mar 3;11(3):656. doi: 10.3390/microorganisms11030656.
9
AMPK Activation Restores Salivary Function Following Radiation Treatment.AMPK 激活可恢复放疗后的唾液功能。
J Dent Res. 2023 May;102(5):546-554. doi: 10.1177/00220345221148983. Epub 2023 Feb 1.
10
Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut.非消化性低聚糖对肠漏的直接作用。
Nutrients. 2022 Nov 7;14(21):4699. doi: 10.3390/nu14214699.
本文引用的文献
1
gut culture for studying differentiation and migration of small intestinal epithelial cells.用于研究小肠上皮细胞分化和迁移的肠道培养。
Open Biol. 2018 Apr;8(4). doi: 10.1098/rsob.170256.
2
Phytosomal-curcumin antagonizes cell growth and migration, induced by thrombin through AMP-Kinase in breast cancer.姜黄素纳米脂质体通过 AMP 激酶拮抗凝血酶诱导的乳腺癌细胞生长和迁移。
J Cell Biochem. 2018 Jul;119(7):5996-6007. doi: 10.1002/jcb.26796. Epub 2018 Mar 30.
3
Metformin inhibits inflammatory signals in the gut by controlling AMPK and p38 MAP kinase activation.二甲双胍通过控制 AMPK 和 p38 MAP 激酶的激活来抑制肠道中的炎症信号。
Clin Sci (Lond). 2018 Jun 12;132(11):1155-1168. doi: 10.1042/CS20180167. Print 2018 Jun 15.
4
Junctional adhesion molecule-A: functional diversity through molecular promiscuity.连接黏附分子-A：分子混杂的功能多样性。
Cell Mol Life Sci. 2018 Apr;75(8):1393-1409. doi: 10.1007/s00018-017-2729-0. Epub 2017 Dec 14.
5
Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers.连接黏附分子A的失调导致乙醇诱导的肠上皮细胞单层屏障破坏。
Physiol Rep. 2017 Dec;5(23). doi: 10.14814/phy2.13541.
6
Purple Potato Extract Promotes Intestinal Epithelial Differentiation and Barrier Function by Activating AMP-Activated Protein Kinase.紫薯提取物通过激活 AMP 激活的蛋白激酶促进肠道上皮细胞分化和屏障功能。
Mol Nutr Food Res. 2018 Feb;62(4). doi: 10.1002/mnfr.201700536. Epub 2018 Jan 25.
7
Dietary red raspberries attenuate dextran sulfate sodium-induced acute colitis.饮食中的红树莓可减轻葡聚糖硫酸钠诱导的急性结肠炎。
J Nutr Biochem. 2018 Jan;51:40-46. doi: 10.1016/j.jnutbio.2017.08.017. Epub 2017 Sep 14.
8
Junctional Adhesion Molecules (JAMs): Cell Adhesion Receptors With Pleiotropic Functions in Cell Physiology and Development.连接黏附分子（JAMs）：在细胞生理学和发育中具有多种功能的细胞黏附受体。
Physiol Rev. 2017 Oct 1;97(4):1529-1554. doi: 10.1152/physrev.00004.2017.
9
Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1.间充质基质细胞通过AMP激活的蛋白激酶途径加速上皮紧密连接组装，独立于肝激酶B1。
Stem Cells Int. 2017;2017:9717353. doi: 10.1155/2017/9717353. Epub 2017 Jul 11.
10
Tricellulin is regulated via interleukin-13-receptor α2, affects macromolecule uptake, and is decreased in ulcerative colitis.三肽基胞苷通过白细胞介素-13 受体 α2 调控，影响大分子摄取，并在溃疡性结肠炎中减少。
Mucosal Immunol. 2018 Mar;11(2):345-356. doi: 10.1038/mi.2017.52. Epub 2017 Jun 14.
Zotero 插件