Zhu Mei-Jun, Sun Xiaofei, Du Min
a School of Food Science , Washington State University , Pullman , WA, USA.
b Department of Animal Sciences , Washington State University , Pullman , WA, USA.
Tissue Barriers. 2018;6(2):1-13. doi: 10.1080/21688370.2018.1487249. Epub 2018 Aug 21.
Gut epithelium covers the inner layer of the gastrointestinal tract and provides a physical barrier to separate the host from its external environment, and its barrier function is critical for maintaining host health. AMP-activated protein kinase (AMPK) as a master regulator of energy metabolism plays a critical role in epithelial barrier function. AMPK activation promotes epithelial differentiation and facilitates cell polarity establishment, both of which strengthen epithelial barrier. In addition, AMPK promotes the assembly of tight junctions and adherens junctions by direct phosphorylation of proteins composing apical junctions, junctional anchors, and cytoskeletons. Pharmacological and nutraceutical compounds, as well as physiological states triggering AMPK activation strengthen epithelial barrier function. This review summarized recent progress in delineating the regulatory roles of AMPK in apical junction formation and barrier function of intestinal epithelium.
肠道上皮覆盖胃肠道内层，提供物理屏障以将宿主与其外部环境分隔开，其屏障功能对于维持宿主健康至关重要。AMP激活的蛋白激酶（AMPK）作为能量代谢的主要调节因子，在上皮屏障功能中起关键作用。AMPK激活促进上皮分化并有助于细胞极性建立，这两者都能加强上皮屏障。此外，AMPK通过直接磷酸化构成顶端连接、连接锚定和细胞骨架的蛋白质来促进紧密连接和黏附连接的组装。药理和营养化合物以及触发AMPK激活的生理状态可增强上皮屏障功能。本综述总结了在阐明AMPK在肠道上皮顶端连接形成和屏障功能中的调节作用方面的最新进展。
