质粒消除和插入序列介导的基因丢失
Loss of Genes Mediated by Plasmid Elimination and IS.
机构信息
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Key Laboratory of Food Safety Risk Assessment, National Health Commission of the People's Republic of China, China National Center for Food Safety Risk Assessment, Beijing, People's Republic of China.
出版信息
Antimicrob Agents Chemother. 2019 Aug 23;63(9). doi: 10.1128/AAC.01002-19. Print 2019 Sep.
Abstract
We characterized the stability of a plasmid pCP53-mcr1_3 encoding and with and without colistin exposure during cultural passages via S1-pulsed-field gel electrophoresis (PFGE) and nanopore MinION sequencing. Both and were missing in certain subclones, mediated by genetic excision (IS), and deletions of large multidrug resistance (MDR) regions confirmed by IS and plasmid elimination. Without colistin exposure, the eradication of genes is feasible, while the factors influencing the elimination processes warrant further study.
摘要
我们通过 S1 脉冲场凝胶电泳（PFGE）和纳米孔 MinION 测序来表征携带 mcr-1_3 基因和/或经多轮传代后质粒稳定性。遗传切除（IS）介导的特定亚克隆中缺失 和 ，且大的多重耐药（MDR）区域缺失通过 IS 和质粒消除得以证实。未经多粘菌素暴露， 基因消除是可行的，而影响消除过程的因素则需要进一步研究。
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