a Department of Cardiology , 2nd Affiliated Hospital of Harbin Medical University, and the Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education , Harbin , People's Republic of China.
b Department of Intensive Care Unit , Ningbo No 2 Hospital , Ningbo , People's Republic of China.
Drug Deliv. 2019 Dec;26(1):612-621. doi: 10.1080/10717544.2019.1628117.
Our study aims to investigate the effect of microRNA-183 (miR-183) on human umbilical vascular endothelial cells (HUVECs) injury by targeting IRS1. HUVECs injury was induced by oxidized low-density lipoprotein (ox-LDL). HUVECs were grouped so as to explore the role of ox-LDL and miR-183 in HUVECs injury, with the expression of miR-183 and IRS1 detected. Additionally, the related factors of oxidative stress and inflammation, as well as angiogenesis ability, proliferation, cell cycle, apoptosis, invasion, and migration abilities were also measured. Ox-LDL treatment could decrease the activity of HUVECs, increase the level of oxidative stress and inflammation, and induce the HUVECs injury. miR-183 could inhibit the expression of IRS1. The inhibition of miR-183 expression in ox-LDL-induced HUVECs injury could enhance cell activity, inhibit inflammatory level, and thus resist cell injury. Low expression of IRS1 could reverse the inhibition of miR-183 on HUVECs injury. This study highlights that inhibition of miR-183 expression may resist HUVECs injury by upregulating expression of IRS1.
本研究旨在探讨 microRNA-183（miR-183）通过靶向 IRS1 对人脐静脉内皮细胞（HUVEC）损伤的影响。氧化型低密度脂蛋白（ox-LDL）诱导 HUVEC 损伤。将 HUVEC 分组，以探讨 ox-LDL 和 miR-183 在 HUVEC 损伤中的作用，检测 miR-183 和 IRS1 的表达。此外，还测量了氧化应激和炎症的相关因素以及血管生成能力、增殖、细胞周期、凋亡、侵袭和迁移能力。ox-LDL 处理可降低 HUVEC 的活性，增加氧化应激和炎症水平，诱导 HUVEC 损伤。miR-183 可抑制 IRS1 的表达。ox-LDL 诱导的 HUVEC 损伤中 miR-183 表达的抑制可增强细胞活性，抑制炎症水平，从而抵抗细胞损伤。IRS1 的低表达可逆转 miR-183 对 HUVEC 损伤的抑制作用。本研究强调，抑制 miR-183 的表达可能通过上调 IRS1 的表达来抵抗 HUVEC 损伤。
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