Engelberth Marie, Selman Samantha M, Engelberth Jurgen
Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Plants (Basel). 2019 Jun 11;8(6):165. doi: 10.3390/plants8060165.
Green leaf volatiles (GLVs), which have mostly been described as providers of protection against insect herbivory and necrotrophic pathogen infections, were recently shown to provide significant fortification against cold stress damage. It was further demonstrated that cold-damaged maize seedlings released a significant amount of GLVs, in particular, Z-3-hexenal (Z-3-HAL). Here, we report that the in-cold treatment of maize seedlings with Z-3-HAL significantly improved cold stress resistance. The transcripts for cold-protective genes were also significantly increased in the Z-3-HAL treated maize seedlings over those found in only cold stressed plants. Consequently, the maize seedlings treated with HAL during cold showed a significantly increased structural integrity, significantly less damage, and increased growth after cold stress, relative to the non-HAL treated maize seedlings. Together, these results demonstrate the protective effect of in-cold treatment with HAL against cold damage, and suggest that the perception of these compounds during cold episodes significantly improves resistance against this abiotic stress.
绿叶挥发物（GLVs）大多被描述为可抵御昆虫食草和坏死性病原菌感染的物质，最近研究表明其能显著增强对冷胁迫损伤的防御能力。进一步的研究表明，受冷害的玉米幼苗会释放大量的绿叶挥发物，尤其是顺-3-己烯醛（Z-3-HAL）。在此，我们报告用Z-3-HAL对玉米幼苗进行冷处理可显著提高其抗冷胁迫能力。与仅受冷胁迫的植株相比，经Z-3-HAL处理的玉米幼苗中抗冷保护基因的转录本也显著增加。因此，与未用HAL处理的玉米幼苗相比，在冷处理期间用HAL处理的玉米幼苗在冷胁迫后显示出结构完整性显著增强、损伤显著减少且生长加快。这些结果共同证明了在冷处理中使用HAL对冷害的保护作用，并表明在冷胁迫期间对这些化合物的感知可显著提高对这种非生物胁迫的抗性。
