Vioti Geovanna, Leonel João Augusto Franco, Lemes Kleber Menegon, Pereira Vanessa Figueredo, Ferreira Helena Lage, Keid Lara Borges, Madureira Ed Hoffman, Soares Rodrigo Martins, Benassi Júlia Cristina, Oliveira Trícia Maria Ferreira de Sousa
Programa de Pós-graduação em Epidemiologia Experimental Aplicada às Zoonoses, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, Pirassununga, SP, Brasil.
Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, Pirassununga, SP, Brasil.
Rev Bras Parasitol Vet. 2019 Jun 13;28(2):303-305. doi: 10.1590/S1984-29612019003.
Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.
利什曼原虫属是引起人类和动物利什曼病的重要病原体，对公共卫生有重要影响。在本研究中，我们旨在检测巴西圣保罗州一个内脏利什曼病非流行区牛群中利什曼原虫属的传播情况。从皮拉苏农加市100头小母牛的血液样本中提取DNA，并使用针对第一个内部转录间隔区（ITS1）的特异性引物进行扩增，以评估锥虫的存在情况。检测结果显示，一个样本出现了300至350个碱基对的条带。在GenBank中，该样本与婴儿利什曼原虫（314个碱基对）的匹配度为100%。结果表明，在巴西牛群可能会感染婴儿利什曼原虫。
