Leonel João Augusto Franco, Vioti Geovanna, Alves Maria Luana, Spada Julio Cesar Pereira, Yamaguchi Alex Kazuo, Pereira Nuno Wolfgang Balbini, da Silva Diogo Tiago, Benassi Julia Cristina, Galvis-Ovallos Fredy, Galati Eunice Aparecida Bianchi, Starke-Buzetti Wilma Aparecida, Soares Rodrigo Martins, Fukumasu Heidge, Oliveira Trícia Maria Ferreira de Sousa
Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo-USP, Pirassununga, State of São Paulo, Brazil.
Post-Graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo-USP, Pirassununga, State of São Paulo, Brazil.
Transbound Emerg Dis. 2024 Feb 22;2024:9932530. doi: 10.1155/2024/9932530. eCollection 2024.
Leishmaniases are important neglected tropical diseases caused by protozoans of the genus spp. The female sandflies bite (Diptera: Psychodidae: Phlebotominae) is the main transmission form in the epidemiological chains of these diseases. Thus, studies on Phlebotominae subfamily are fundamental to improve the comprehension of the leishmaniases epidemiology, revealing relationships of these dipterans with the parasite and reservoirs. An entomological survey of Phlebotomine sandflies was carried out to investigate the fauna, spp. DNA detection and blood meals sources of female sandflies captured in peridomiciles areas from a leishmaniases endemic area of São Paulo state, Brazil. A total of 637 Phlebotomine sandflies specimens were captured, and twelve species identified, some of them proven or suspected vectors of tegumentary leishmaniasis (TL) and visceral leishmaniasis (VL). () and () PCR positive to () kinetoplast minicircle DNA (kDNA) were identified. Also, () kDNA was found in () and . (.) . Regarding blood meals source, DNA of swine, humans, dogs, cattle, chickens, and opossums were detected in the midgut of Phlebotomine sandflies females captured in the study area. Our results highlight ecological relationships among different species of Phlebotomine sandflies, domestic and wild-synanthropic vertebrates (including humans) and two species in peridomiciles from a leishmaniases endemic area of Brazil.
利什曼病是由利什曼原虫属原生动物引起的重要的被忽视的热带病。雌性白蛉（双翅目：蛾蠓科：白蛉亚科）叮咬是这些疾病流行病学传播链中的主要传播形式。因此，对白蛉亚科的研究对于增进对利什曼病流行病学的理解、揭示这些双翅目昆虫与寄生虫及宿主的关系至关重要。在巴西圣保罗州一个利什曼病流行地区的住宅周围区域，对白蛉进行了一项昆虫学调查，以研究其种类、利什曼原虫属物种DNA检测以及捕获的雌性白蛉的血餐来源。共捕获637只白蛉标本，鉴定出12个物种，其中一些被证实或怀疑是皮肤利什曼病（TL）和内脏利什曼病（VL）的传播媒介。鉴定出对利什曼原虫动质小环DNA（kDNA）呈PCR阳性的（）和（）。此外，在（）和中发现了（）kDNA。（.）。关于血餐来源，在研究区域捕获的雌性白蛉中肠中检测到猪、人、狗、牛、鸡和负鼠的DNA。我们的结果突出了巴西一个利什曼病流行地区住宅周围不同种类白蛉、家养和野生共栖脊椎动物（包括人类）以及两种利什曼原虫之间的生态关系。
