质子磁共振波谱法检测心肌缺血再灌注损伤大鼠脊髓氨基酸水平及代谢物比值的变化
Alterations in amino acid levels and metabolite ratio of spinal cord in rat with myocardial ischemia-reperfusion injury by proton magnetic resonance spectroscopy.
作者信息
Wang Qian, Li Zhi-Xiao, Li Yu-Juan, Manyande Anne, Li Shun-Yuan, Feng Mao-Hui, Wu Duo-Zhi, Xiang Hong-Bing
机构信息
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, PR China.
School of Human and Social Sciences, University of West London London, UK.
出版信息
Am J Transl Res. 2019 May 15;11(5):3101-3108. eCollection 2019.
OBJECTIVES
The mechanism behind spinal metabolites and myocardial ischemia-reperfusion (IR) injury is not well understood. Proton magnetic resonance spectroscopic analysis of spinal cord extracts provides a quick evaluation of the specific metabolic activity in rats with myocardial IR injury. We investigated the relationship between the IR-related variables and the changes in spinal metabolites.
METHODS
Proton magnetic resonance spectroscopy (H-MRS) was used to assess the spinal metabolites of adult rats with and without myocardial IR injury (n = 6 per group). Myocardial IR injury was reproduced using intermittent occlusion of the left anterior descending coronary artery. We studied the relationship between the metabolite ratio measurement and IR-related variables. All rats underwent H-MRS, with the ratio of interest placed in different spinal cord segments to measure levels of twelve metabolites including N-acetylaspartate (NAA), taurine (Tau), glutamate (Glu), gamma amino acid butyric acid (GABA), creatine (Cr), and myoinositol (MI), etc. Results: Rats with myocardial IR injury had higher concentration of Tau in the upper thoracic spinal cord (P < 0.05), and lower concentration of Gly and Glu in the cervical segment of the spinal cord (P < 0.05), when compared to the Control group. The ratios of glutamate/taurine (Glu/Tau), Glu/(GABA + Tau) and Glu/Total were significantly different between the IR group and the Control group in the upper thoracic spinal cord (P < 0.05). So were the ratios of Glu/(GABA + Tau) in the cervical segment (P < 0.05), and Glu/Tau and Glu/(GABA + Tau) in the lower thoracic spinal cord (P < 0.05).
CONCLUSIONS
These findings suggest that myocardial IR injury may be related to spinal biochemical alterations. It is speculated that these observed changes in the levels of spinal metabolites may be involved in the pathogenesis and regulation of myocardial IR injury.
目的
脊髓代谢物与心肌缺血再灌注（IR）损伤背后的机制尚未完全明确。对脊髓提取物进行质子磁共振波谱分析可快速评估心肌IR损伤大鼠的特定代谢活性。我们研究了IR相关变量与脊髓代谢物变化之间的关系。
方法
采用质子磁共振波谱（H-MRS）评估有无心肌IR损伤的成年大鼠（每组n = 6）的脊髓代谢物。通过间歇性阻断左前降支冠状动脉来复制心肌IR损伤。我们研究了代谢物比率测量与IR相关变量之间的关系。所有大鼠均接受H-MRS检查，将感兴趣的比率置于不同脊髓节段以测量包括N-乙酰天门冬氨酸（NAA）、牛磺酸（Tau）、谷氨酸（Glu）、γ-氨基丁酸（GABA）、肌酸（Cr）和肌醇（MI）等十二种代谢物的水平。结果：与对照组相比，心肌IR损伤大鼠上胸段脊髓中Tau浓度较高（P < 0.05），脊髓颈段Gly和Glu浓度较低（P < 0.05）。IR组与对照组在上胸段脊髓中谷氨酸/牛磺酸（Glu/Tau）、Glu/（GABA + Tau）和Glu/总量的比率有显著差异（P < 0.05）。颈段的Glu/（GABA + Tau）比率（P < 0.05）以及下胸段脊髓的Glu/Tau和Glu/（GABA + Tau）比率（P < 0.05）也有显著差异。
结论
这些发现表明心肌IR损伤可能与脊髓生化改变有关。据推测，脊髓代谢物水平的这些观察到的变化可能参与了心肌IR损伤的发病机制和调节。
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