Ishaq Razia, Ansari Muhammad S, Rakha Bushra A, Qadeer Saima, Akhter Shamim
Department of Zoology, Government College Women University, Sialkot, Pakistan.
Division of Science and Technology, University of Education, Lahore, Pakistan.
Biopreserv Biobank. 2019 Dec;17(6):546-552. doi: 10.1089/bio.2018.0133. Epub 2019 Jun 20.
The present study was designed to evaluate enrofloxacin (EN), a fluoroquinolone broad-spectrum antibacterial drug for use in cryopreservation of Zebu bull semen. Semen was collected from three Zebu bulls of the Sahiwal breed () for 3 weeks (replicates) for each experiment. Experiment I: semen samples were cultured for isolation of bacteria and sensitivity tests against streptomycin, penicillin, and EN. spp., , , and were isolated. was found to be resistant to streptomycin and penicillin (SP), while spp. was resistant to streptomycin. EN effectively inhibited the growth of all four bacteria in an sensitivity test. Experiment II: dose toxicity of EN (0-2000 μg/mL) for bull sperm was assessed in 2.9% sodium citrate buffer. EN up to a concentration of 1200 μg/mL was found to be safe for motility and viability of bull spermatozoa. Experiment III: semen was cryopreserved in a -citric acid extender containing either SP or EN (400 μg/mL) or without antibiotics as the control. Post-thaw sperm motility was found to be higher in the extender containing EN and SP compared to control. Nevertheless, sperm viability was recorded higher ( < 0.05) with EN compared to SP and control. Sperm chromatin integrity did not differ ( > 0.05) in EN, SP, and controls. The total aerobic bacterial count was recorded as zero in the extender containing EN compared to SP and control. The fertility rate was higher ( < 0.05) with 400 μg/mL EN (59%) compared to SP (44%). It is concluded that EN is capable of controlling the bacterial load in frozen Zebu bull semen, resulting in a higher fertility rate under field conditions.
本研究旨在评估恩诺沙星（EN），一种氟喹诺酮类广谱抗菌药物，用于泽布公牛精液的冷冻保存。在每个实验中，从三头萨希瓦尔品种的泽布公牛采集精液，为期3周（重复实验）。实验I：对精液样本进行细菌培养，以分离细菌并进行对链霉素、青霉素和恩诺沙星的敏感性测试。分离出了金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和铜绿假单胞菌。发现金黄色葡萄球菌对链霉素和青霉素（SP）耐药，而大肠杆菌对链霉素耐药。在药敏试验中，恩诺沙星有效抑制了所有四种细菌的生长。实验II：在2.9%柠檬酸钠缓冲液中评估恩诺沙星（0 - 2000μg/mL）对公牛精子的剂量毒性。发现浓度高达1200μg/mL的恩诺沙星对公牛精子的活力和生存力是安全的。实验III：精液在含有SP或恩诺沙星（400μg/mL）或不含抗生素作为对照的柠檬酸盐-柠檬酸缓冲液中进行冷冻保存。解冻后，发现含有恩诺沙星和SP的缓冲液中的精子活力高于对照组。然而，与SP和对照组相比，恩诺沙星组的精子生存力更高（P < 0.05）。恩诺沙星组、SP组和对照组的精子染色质完整性没有差异（P > 0.05）。与SP组和对照组相比，含有恩诺沙星的缓冲液中的需氧细菌总数记录为零。与SP组（44%）相比，400μg/mL恩诺沙星组的受胎率更高（P < 0.05）（59%）。得出的结论是，恩诺沙星能够控制冷冻泽布公牛精液中的细菌载量，从而在田间条件下提高受胎率。
