Germanyuk Y L, Minchenko A G
Endocrinol Exp. 1978 Dec;12(4):233-43.
In liver mitochondria from alloxan diabetic rats the biosynthesis of RNA (as measured by 14C-orotic acid incorporation and RNA polymerase activity) and protein (as measured by 14C-leucine incorporation) were decreased. In contrast, insulin (20 U kg-1) injected to intact or diabetic rats increased both these measures. However, no change of mitochondrial DNA biosynthesis (as measured by incorporation of 3H-thymidine) was found. It was concluded that in liver cells insulin plays an important role in regulation of biosynthesis not only of nuclear (cytoplasmic) but also of mitochondrial RNA and proteins.
在四氧嘧啶糖尿病大鼠的肝脏线粒体中，RNA的生物合成（通过14C-乳清酸掺入和RNA聚合酶活性来测定）和蛋白质的生物合成（通过14C-亮氨酸掺入来测定）均减少。相比之下，给完整或糖尿病大鼠注射胰岛素（20 U/kg-1）可使这两项指标均升高。然而，未发现线粒体DNA生物合成（通过3H-胸腺嘧啶掺入来测定）有变化。得出的结论是，在肝细胞中，胰岛素不仅在调节细胞核（细胞质）的生物合成中起重要作用，而且在调节线粒体RNA和蛋白质的生物合成中也起重要作用。
