Bréant B, Chamblier M C, Rosselin G
C R Acad Sci III. 1984;299(14):571-4.
We examined the effect of diabetes on protein synthesis in intestinal epithelial cells. Isolated enterocytes of diabetic rats display a marked decrease (50%) in 3H-leucine incorporation into intestinal proteins as compared to normal cells. Treatment of diabetics with insulin restores the level of protein synthesis up to normal values. Similarly, in vitro translation products of isolated RNA are much lower in diabetics than in normal or treated diabetics. It is concluded that in intestinal cells, insulin can play an important role in regulating the biosynthesis of proteins by modulating the translation of specific RNAs.
我们研究了糖尿病对肠上皮细胞蛋白质合成的影响。与正常细胞相比，糖尿病大鼠分离出的肠上皮细胞中，3H-亮氨酸掺入肠蛋白的量显著减少（50%）。用胰岛素治疗糖尿病大鼠可使蛋白质合成水平恢复至正常。同样，分离出的RNA的体外翻译产物在糖尿病大鼠中比正常或经治疗的糖尿病大鼠低得多。得出的结论是，在肠细胞中，胰岛素可通过调节特定RNA的翻译在调节蛋白质生物合成中发挥重要作用。
