Anderson L C, Shapiro B L
Horm Metab Res. 1980 Feb;12(2):47-50. doi: 10.1055/s-2007-996198.
The incorporation of radioactively labeled leucine into TCA-precipitable proteins by submandibular gland tissue slices from control, alloxan diabetic, and insulin supplemented diabetic rats was measured in vitro. Incorporation decreased in alloxan diabetes and could be restored to control levels within three hours after insulin administration. The effects of alloxan diabetes and insulin on 3H-leucine incorporation paralleled their effects on a secretory enzyme, peroxidase. Insulin in vitro stimulated the incorporation of 3H-leucine within 15 minutes of addition to the incubation medium. Further, the response to insulin was found to be dose-related. The conclusion drawn from these results is that insulin has a rapid, direct effect on the rate of protein synthesis in the rat submandibular gland.
通过体外测量对照大鼠、四氧嘧啶糖尿病大鼠以及补充胰岛素的糖尿病大鼠的下颌下腺组织切片将放射性标记的亮氨酸掺入三羧酸循环可沉淀蛋白中的情况。四氧嘧啶糖尿病时掺入量减少，胰岛素给药后三小时内可恢复到对照水平。四氧嘧啶糖尿病和胰岛素对3H-亮氨酸掺入的影响与其对一种分泌酶过氧化物酶的影响相似。体外胰岛素在添加到孵育培养基后15分钟内刺激3H-亮氨酸的掺入。此外，发现对胰岛素的反应与剂量相关。从这些结果得出结论，胰岛素对大鼠下颌下腺蛋白质合成速率有快速、直接的作用。
