Department of Restorative Dentistry, Federal University of Piaui, Teresina, Brazil.
Department of Mechanical Engineering, Federal University of Piaui, Teresina, Brazil.
Gerodontology. 2019 Dec;36(4):345-351. doi: 10.1111/ger.12419. Epub 2019 Jun 20.
To evaluate root dentine demineralisation, biomass and loosely bound fluoride (CaF ) concentration according to different frequencies of sucrose exposure using a high-fluoride dentifrice.
Although high-fluoride dentifrice has been recommended to arrest root dentine lesions, it is not clear whether it can protect dentine from increased frequencies of sucrose exposure.
An in situ, crossover, split-mouth study was conducted in 3 phases with 7 days each, in which 10 volunteers used a palatal device containing 4 bovine root dentine slabs (2 on each side) with predetermined initial hardness. Cariogenic challenge consisted in dripping a 20% sucrose solution 0 (control), 2, 4, 6, 8 or 10 times/d in each block. Volunteers used high-fluoride dentifrice (NaF, 5000 µg F/g) 3 times/d. After each phase, final hardness was measured and the percentage of surface hardness loss (%SHL) calculated. Also, biomass and CaF concentration on dentine were determined. The data were processed and analysed by ANOVA and Tukey test with significance level set at 5%. The relationship between the variables was analysed by linear regression and Pearson correlation (r).
%SHL and biomass were significantly greater than control for sucrose frequencies higher than 6 times/d (P < 0.001), while CaF concentration decreased from sucrose frequency higher than 2 times/d (P < 0.001). Regression analysis data showed a significant linear fit between sucrose exposure frequency and the studied variables with a strong correlation (r) for %SHL and CaF and moderate for biomass (P < 0.05).
High-fluoride dentifrice is able to reduce root dentine demineralisation if sucrose consumption is not higher than 6 times/d.
使用高氟牙膏，评估不同蔗糖暴露频率下根牙本质脱矿、生物量和松散结合氟（CaF ）浓度。
尽管高氟牙膏已被推荐用于阻止根牙本质病变，但尚不清楚它是否能保护牙本质免受蔗糖暴露频率增加的影响。
采用原位、交叉、劈裂口腔研究，共分 3 个阶段，每个阶段 7 天，共有 10 名志愿者使用含 4 块牛根牙本质板（每侧 2 块）的腭部装置，每块板均具有预定的初始硬度。致龋性挑战包括在每个块上每天滴 20%蔗糖溶液 0（对照）、2、4、6、8 或 10 次。志愿者每天使用高氟牙膏（NaF，5000μg F/g）3 次。每个阶段后，测量最终硬度并计算表面硬度损失百分比（%SHL）。同时，还测定牙本质上的生物量和 CaF 浓度。数据采用 ANOVA 和 Tukey 检验进行处理和分析，显著性水平设为 5%。采用线性回归和 Pearson 相关分析（r）分析变量之间的关系。
蔗糖暴露频率高于 6 次/d 时，%SHL 和生物量显著高于对照组（P<0.001），而 CaF 浓度从蔗糖暴露频率高于 2 次/d 时开始下降（P<0.001）。回归分析数据显示，蔗糖暴露频率与研究变量之间存在显著的线性拟合，%SHL 和 CaF 相关性较强（r），生物量相关性适中（P<0.05）。
如果蔗糖摄入量不高于 6 次/d，高氟牙膏能够减少根牙本质脱矿。
