Nóbrega Diego Figueiredo, Fernández Constanza Estefany, Del Bel Cury Altair Antoninha, Tenuta Livia Maria Andaló, Cury Jaime Aparecido
Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.
Caries Res. 2016;50(2):133-40. doi: 10.1159/000444223. Epub 2016 Mar 19.
The clinical relevance of the frequency of fluoride dentifrice (FD) use on enamel caries is based on evidence. However, the relative effect of FD on reduction of demineralization or enhancement of remineralization is unknown and the effect of frequency on root dentine caries has not been explored. The aim of this double-blind, crossover, in situ study, which was conducted in 4 phases of 14 days each, was to evaluate the relationship between the frequency of FD use and enamel and root dentine de- and remineralization. Eighteen volunteers wore palatal appliances containing enamel and root dentine slabs, either sound or carious. Biofilm accumulation on the slab surface was allowed, and 20% sucrose solution was dripped 3 or 8 times per day on the carious and sound slabs, respectively. Volunteers used FD (1,100 μg F/g) in the frequencies 0 (fluoride-placebo dentifrice), 1, 2 and 3 times per day. The demineralization and remineralization that occurred in sound or carious slabs was estimated by the percentage of surface hardness loss (%SHL) or recovery (%SHR). Loosely (CaF2) and firmly (FAp) bound fluoride concentrations were also determined. The relationship between the variables was analyzed by linear regression. The %SHL, CaF2 and FAp concentrations were a function of the frequency of FD use for enamel and dentine, but the %SHR was a function of the frequency of FD use only for enamel (p < 0.05). The results suggest that demineralization in enamel and root dentine is reduced in proportion to the frequency of FD use, but for remineralization the effect of the frequency of FD use was relevant only to enamel.
使用含氟牙膏（FD）的频率对牙釉质龋的临床相关性是有证据依据的。然而，FD对减少脱矿或增强再矿化的相对作用尚不清楚，且其使用频率对根面牙本质龋的影响尚未得到研究。这项双盲、交叉、原位研究分四个阶段进行，每个阶段为期14天，旨在评估使用FD的频率与牙釉质和根面牙本质脱矿及再矿化之间的关系。18名志愿者佩戴含有牙釉质和根面牙本质片的腭部矫治器，这些牙片要么是健康的，要么是龋坏的。允许在牙片表面形成生物膜，分别每天在龋坏牙片和健康牙片上滴注3次或8次20%的蔗糖溶液。志愿者使用含氟量为1100μg F/g的牙膏，使用频率分别为0次（含氟安慰剂牙膏）、1次、2次和3次/天。通过表面硬度损失百分比（%SHL）或恢复百分比（%SHR）来估算健康或龋坏牙片中发生的脱矿和再矿化情况。还测定了松散结合（CaF2）和紧密结合（FAp）的氟浓度。通过线性回归分析变量之间的关系。%SHL、CaF2和FAp浓度是牙釉质和牙本质使用FD频率的函数，但%SHR仅是牙釉质使用FD频率的函数（p<0.05）。结果表明，牙釉质和根面牙本质的脱矿程度与使用FD的频率成比例降低，但对于再矿化，使用FD的频率仅对牙釉质有影响。
