Department of Restorative Dentistry, Federal University of Piaui, Teresina, Brazil.
Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.
Caries Res. 2020;54(5-6):502-508. doi: 10.1159/000510535. Epub 2020 Oct 19.
The present study aimed to evaluate the effect of fluoride (F) dentifrice with different F concentrations on root dentine de-/remineralization. Ten healthy volunteers took part in this randomized, double-blinded, cross-over, and split-mouth in situ experimental study. During 4 phases of 7 days, they wore a palatal appliance containing 4 bovine dentine blocks (2 sound and 2 with caries) of 4 × 4 × 2 mm. Treatments were performed with silica-based dentifrices containing 0, 700, 1,300, and 5,000 µg F/g (F as NaF). To provide a cariogenic challenge, a 20% sucrose solution was dripped 3 and 8 times daily on the carious-like and sound blocks, respectively. After each experimental phase, the percentage of surface hardness loss (%SHL) or recovery (%SHR) was calculated and the fluoride concentration in the biofilm was determined. The statistical analysis was performed using ANOVA and the Tukey post hoc test with p at 5%. The relationship between variables was analyzed by linear regression. The results showed a lower %SHL when 5,000 µg F/g dentifrice was used but without a statistically significant difference from the conventional one (1,300 µg F/g). Regarding remineralization and F in biofilms, the high-fluoride dentifrice was expressively superior in mineral replacement on the surface and in the F concentration in the biofilms, respectively, compared to the other 3 products (p < 0.05). Also, a significant linear fit between mineral loss/gain, F in biofilms, and fluoride concentration in the dentifrices could be observed. In conclusion, a dose-response F effect was observed, and the high-fluoride dentifrice was effective in enhancing root dentine remineralization in this short-term in situ study.
本研究旨在评估不同氟浓度的氟化物（F）牙膏对根牙本质去/再矿化的影响。 10 名健康志愿者参加了这项随机、双盲、交叉、分口的原位实验研究。 在 4 个为期 7 天的阶段中，他们佩戴了一个包含 4 个牛牙本质块（2 个健康和 2 个龋）的腭器械，尺寸为 4×4×2mm。 使用含有 0、700、1300 和 5000μg F/g（F 为 NaF）的硅基牙膏进行处理。 为了提供致龋挑战，每天在龋样和健康块上分别滴 3 次和 8 次 20%蔗糖溶液。 每个实验阶段后，计算表面硬度损失百分比（%SHL）或恢复百分比（%SHR），并测定生物膜中的氟浓度。 使用方差分析和 Tukey 事后检验进行统计学分析，p 值为 5%。 使用线性回归分析变量之间的关系。 结果表明，使用 5000μg F/g 牙膏时%SHL 较低，但与常规牙膏（1300μg F/g）相比无统计学差异。 关于再矿化和生物膜中的 F，高氟牙膏在表面矿物质替代和生物膜中 F 浓度方面均明显优于其他 3 种产品（p<0.05）。 此外，还可以观察到矿物质损失/增益、生物膜中的 F 和牙膏中的氟浓度之间存在显著的线性拟合。 总之，观察到剂量反应 F 效应，高氟牙膏在这项短期原位研究中有效增强了根牙本质再矿化。
