Nagaoka K, Sakurami T, Nabeya N, Imura H, Kuno S
Endocrinol Jpn. 1979 Feb;26(1):89-95. doi: 10.1507/endocrj1954.26.89.
The leucocyte migration inhibition test (LMT) was performed by the agarose plate method with thyroid and pancreatic antigens in patients with insulin-dependent or independent diabetes mellitus. The mean migration indices with thyroglobulin, thyroid mitochondria and beef insulin were not significantly different in insulin-dependent diabetics from those in insulin-independent diabetics or normal controls. However, significant inhibition of leucocyte migration was observed in insulin-dependent diabetics when thyroid microsome or pancreatic extract was used as antigen. Although no significant difference was found in the percentages of T and B lymphocytes between insulin-dependent diabetics and insulin-independent diabetics or normal controls, the results of LMT strongly suggest the presence of cellular immunity against the thyroid and pancreas in insulin-dependent juvenile-onset diabetes.
采用琼脂平板法，以甲状腺和胰腺抗原对胰岛素依赖型或非胰岛素依赖型糖尿病患者进行白细胞迁移抑制试验（LMT）。胰岛素依赖型糖尿病患者针对甲状腺球蛋白、甲状腺线粒体和牛胰岛素的平均迁移指数与非胰岛素依赖型糖尿病患者或正常对照者相比，无显著差异。然而，当以甲状腺微粒体或胰腺提取物作为抗原时，在胰岛素依赖型糖尿病患者中观察到白细胞迁移受到显著抑制。虽然胰岛素依赖型糖尿病患者与非胰岛素依赖型糖尿病患者或正常对照者之间的T淋巴细胞和B淋巴细胞百分比无显著差异，但LMT结果强烈提示，胰岛素依赖型青少年发病糖尿病患者存在针对甲状腺和胰腺的细胞免疫。
