Nagaoka K, Nabeya N, Sakurami T, Imura H, Kuno S
Nihon Naibunpi Gakkai Zasshi. 1978 Jan 20;54(1):36-42. doi: 10.1507/endocrine1927.54.1_36.
There is an increasing evidence that autoimmune mechanisms may have a role in the pathogenesis in insulin-dependent diabetics. The numerical and functional study of peripheral blood lymphocytes in diabetes mellitus might indirectly contribute to the understanding of its pathogenesis. In this study, detection of peripheral blood T lymphocytes was measured by rosettes with sheep red blood cells (SRBC), and B lymphocytes were measured by immunofluorescence with specific antiserum to immunoglobulins. The mean (+/- SD) percentage of SRBC was 67.6 +/- 7.2 in 21 normal subjects, 71.5 +/- 7.0 in 15 insulin-dependent diabetics, and 68.6 +/- 6.7 in 30 insulin-independent diabetics. There was no difference in the absolute T-lymphocyte number per mm3 in these three groups. Insulin-dependent diabetics showed a normal percentage and absolute number of B lymphocytes when compared with normal subjects.
越来越多的证据表明，自身免疫机制可能在胰岛素依赖型糖尿病的发病机制中起作用。对糖尿病患者外周血淋巴细胞进行数量和功能研究可能会间接有助于理解其发病机制。在本研究中，通过与绵羊红细胞（SRBC）形成玫瑰花结来检测外周血T淋巴细胞，并用针对免疫球蛋白的特异性抗血清通过免疫荧光法检测B淋巴细胞。21名正常受试者中SRBC的平均（±标准差）百分比为67.6±7.2，15名胰岛素依赖型糖尿病患者中为71.5±7.0，30名非胰岛素依赖型糖尿病患者中为68.6±6.7。这三组每立方毫米的绝对T淋巴细胞数量没有差异。与正常受试者相比，胰岛素依赖型糖尿病患者的B淋巴细胞百分比和绝对数量正常。
