Gutiérrez C, Vega J, Kreisler M
Eur J Immunol. 1979 Jan;9(1):72-6. doi: 10.1002/eji.1830090115.
Normal human peripheral blood lymphocytes were shown to activate complement in normal human serum (NHS). This activation led to C3 fixation on the cell membrane, which in turn was visualized by fluorescence. The reaction occurred in the absence of detectable amounts of antibodies, since an agammaglobulinemic patient's serum also supported complement activation, and the results were unaltered by absorption of normal human serum. Trypsin treatment of lymphocytes to remove possible complement activators bound to the cell membrane, did not have any effect on the complement activation by lymphocytes. Complement membrane fluorescence was abolished in the presence of EDTA or ethyleneglycolbis(aminoethylether) tetraacetate (EGTA) supplemented by Mg++ions indicating that the classical pathway of complement activation was involved in the reaction. Experiments performed with T and B-purified populations showed that the phenomenon was confined to the B cell fraction. Complement deposition did not diminish the number of EAC rosettes, and viability remained unchanged after exposure of lymphocytes to normal human serum.
正常人类外周血淋巴细胞可在正常人血清（NHS）中激活补体。这种激活导致C3固定在细胞膜上，进而通过荧光显现出来。该反应在未检测到抗体的情况下发生，因为一名无丙种球蛋白血症患者的血清也能支持补体激活，且正常人血清的吸收对结果无影响。用胰蛋白酶处理淋巴细胞以去除可能结合在细胞膜上的补体激活剂，对淋巴细胞激活补体没有任何影响。在添加Mg++离子的乙二胺四乙酸（EDTA）或乙二醇双（2-氨基乙醚）四乙酸（EGTA）存在的情况下，补体膜荧光消失，表明补体激活的经典途径参与了该反应。对T细胞和B细胞纯化群体进行的实验表明，该现象仅限于B细胞部分。补体沉积并未减少EAC玫瑰花结的数量，淋巴细胞暴露于正常人血清后活力保持不变。
