Martin M J, Vega J, Gutiérrez C
Immunology. 1980 Nov;41(3):535-9.
A study was made of the complement activation (CA) capacity of human T lymphoblasts obtained in vitro by PHA stimulation of highly purified T cells. The CA was studied by immunofluorescent detection of the C3 fragments deposited onto the cell membrane following incubation in autologous normal human serum (NHS). Whereas resting T cells did not stain, a mean of 12% of the blast cells were positive for monospecific anti-human C3 fluorescent serum. The possible mechanism through which C3 was deposited on the cell membrane may be a consequence of CA, because complement membrane fluorescence (CMF) was abolished by chelation of divalent cations with EDTA, complement inactivation by heating or incubation at low temperature. This reaction appears to occur in the absence of antibodies, because similar results were found when human agammaglobulinaemic serum was used as the complement source. The addition of ethyleneglycolbis-(aminoethylether)-tetraacetate (EGTA), supplemented with MgCl2 to the NHS failed to abolish the fluorescence, indicating that the alternative pathway was involved in the phenomenon. It is likely that the capacity to activate complement may distinguish a functional subpopulation of human T lymphoblasts.
对通过PHA刺激高度纯化的T细胞体外获得的人T淋巴母细胞的补体激活（CA）能力进行了研究。通过免疫荧光检测在自体正常人血清（NHS）中孵育后沉积在细胞膜上的C3片段来研究CA。静止的T细胞不染色，而平均12%的母细胞对单特异性抗人C3荧光血清呈阳性。C3沉积在细胞膜上的可能机制可能是CA的结果，因为通过用EDTA螯合二价阳离子、加热或在低温下孵育使补体失活，补体膜荧光（CMF）被消除。这种反应似乎在没有抗体的情况下发生，因为当使用人无丙种球蛋白血症血清作为补体来源时发现了类似的结果。向NHS中添加补充有MgCl2的乙二醇双（氨基乙基醚）四乙酸（EGTA）未能消除荧光，表明替代途径参与了该现象。激活补体的能力可能区分人T淋巴母细胞的一个功能亚群。
