Gordon J, Robinson D S, Stevenson G T
Immunology. 1981 Jan;42(1):7-12.
Following previous authors, the term antigenic modulation is used to describe the induction, by antibody, of resistance to lysis by antibody plus complement. A report is given of the rapid antigenic modulation in vitro of surface immunoglobulin (Ig) on guinea-pig L2C leukaemic lymphocytes: incubation of the cells for 2 min or longer at 37 degrees with anti-Ig diminished or removed completely the lysis occurring during subsequent incubation with anti-Ig plus complement. The modulation was effective for both xenogeneic (rabbit) and syngeneic (guinea-pig strain 2) complements, but more rapid for the latter. It appeared simply to require the action of antibody on a metabolically active cell: no requirement could be demonstrated for any serum component other than antibody, and there was a need to raise the temperature to 37 degrees after attachment of the antibody. There was molecular specificity inasmuch as modulation with anti-Ig failed to confer any resistance to lysis by another antibody (anti-Ia) plus complement.
沿用先前作者的说法,术语“抗原调制”用于描述抗体诱导产生对抗体加补体介导的细胞溶解的抗性。本文报道了豚鼠L2C白血病淋巴细胞表面免疫球蛋白(Ig)在体外的快速抗原调制:细胞在37℃与抗Ig孵育2分钟或更长时间,可减少或完全消除后续与抗Ig加补体孵育期间发生的细胞溶解。这种调制对异种(兔)和同基因(豚鼠2系)补体均有效,但对后者起效更快。这似乎仅仅需要抗体作用于代谢活跃的细胞:除抗体外,未证明需要任何血清成分,并且在抗体附着后需要将温度升至37℃。存在分子特异性,因为用抗Ig进行调制未能赋予对另一种抗体(抗Ia)加补体介导的细胞溶解的任何抗性。