ICAR-National Bureau of Agriculturally Important Microorganisms, Kusmaur, Mau, Uttar Pradesh, 275101, India.
Department of Postharvest and Food Sciences, The Volcani Center, Agricultural Research Organization, 68 HaMaccabim Road, 7505101, Rishon LeZion, Israel.
Antonie Van Leeuwenhoek. 2019 Nov;112(11):1633-1644. doi: 10.1007/s10482-019-01290-8. Epub 2019 Jun 20.
Biological control by using microbial inoculants is adopted as the best alternative to chemical pesticides to manage plant diseases. In the present study, a microbial consortia based management strategy involving the microbes Bacillus velezensis MB101 (BV), Streptomyces atrovirens N23 (SA) and Trichoderma lixii NAIMCC-F-01760 (TL), was evaluated for the management of Rhizoctonia solani (RS), the causal agent of tomato root rot. The efficacy of these microbial inoculants was evaluated in glasshouse and field experiments. Plant defense-related enzymes were assayed in the glasshouse, and biocontrol effect was evaluated in the field with RS infected soil. In the glasshouse experiment, co-inoculated SA + TL treated plants showed maximum disease resistance in comparison to control. Also, the plant defense-related enzymes such as chitinase, β-1,3-glucanase, peroxidases, polyphenol oxidase, and phenylalanine ammonia lyase were increased in this treatment. Furthermore, three application methods were assessed in the field, and SA + TL showed maximum disease reduction (76%) by the dual application. Based on glasshouse and field study results, it was concluded that co-inoculation of SA + TL activated plant defense against RS as compared to the individual microbes, and co-inoculation could be a new effective strategy to manage the root rot pathogen in an eco-compatible manner.
采用微生物接种剂进行生物防治被视为管理植物病害的最佳替代化学农药的方法。在本研究中，评估了一种基于微生物群落的管理策略，涉及微生物解淀粉芽孢杆菌 MB101（BV）、深绿链霉菌 N23（SA）和木霉菌 NAIMCC-F-01760（TL），用于防治番茄根腐病的病原菌立枯丝核菌（RS）。在温室和田间试验中评估了这些微生物接种剂的功效。在温室中测定了与植物防御相关的酶，并用感染 RS 的土壤在田间评估了生物防治效果。在温室试验中，与对照相比，共同接种 SA+TL 处理的植物表现出最大的抗病性。此外，在这种处理中还增加了几丁质酶、β-1,3-葡聚糖酶、过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶等与植物防御相关的酶。此外，还评估了三种田间应用方法，SA+TL 通过双重应用表现出最大的病害减少（76%）。基于温室和田间研究结果得出结论，与单独使用微生物相比，SA+TL 的共同接种可激活植物对 RS 的防御，共同接种可能是一种新的有效策略，可生态兼容的方式管理根腐病病原体。
