Bio-Protection Research Centre, PO Box 85084, Lincoln University, Lincoln, New Zealand.
Bio-Protection Research Centre, PO Box 85084, Lincoln University, Lincoln, New Zealand.
Genomics. 2020 Jan;112(1):1042-1053. doi: 10.1016/j.ygeno.2019.06.020. Epub 2019 Jun 19.
Brevibacillus laterosporus is a globally ubiquitous, spore forming bacterium, strains of which have shown toxic activity against invertebrates and microbes and several have been patented due to their commercial potential. Relatively little is known about this bacterium. Here, we examined the genomes of six published and five newly determined genomes of B. laterosporus, with an emphasis on the relationships between known and putative toxin encoding genes, as well as the phylogenetic relationships between strains. Phylogenetically, strain relationships are similar using average nucleotide identity (ANI) values and multi-gene approaches, although PacBio sequencing revealed multiple copies of the 16S rDNA gene which lessened utility at the strain level. Based on ANI values, the New Zealand isolates were distant from other isolates and may represent a new species. While all of the genomes examined shared some putative toxicity or virulence related proteins, many specific genes were only present in a subset of strains.
短芽孢杆菌是一种在全球范围内普遍存在的产芽孢细菌，其菌株已显示出对无脊椎动物和微生物的毒性活性，并且由于其商业潜力，已有几种被授予了专利。关于这种细菌，人们了解得相对较少。在这里，我们研究了 6 个已发表的和 5 个新确定的短芽孢杆菌基因组，重点关注已知和推测的毒素编码基因之间的关系，以及菌株之间的系统发育关系。从系统发育上看，使用平均核苷酸同一性 (ANI) 值和多基因方法，菌株之间的关系相似，尽管 PacBio 测序显示 16S rDNA 基因有多个拷贝，这在菌株水平上降低了其可用性。根据 ANI 值，新西兰分离株与其他分离株相距甚远，可能代表一个新物种。虽然所有研究的基因组都共享一些推测的毒性或毒力相关蛋白，但许多特定基因仅存在于一部分菌株中。
